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林产化学与工业 ›› 2008, Vol. 28 ›› Issue (5): 51-54.

• 研究报告 • 上一篇    下一篇

毛竹叶抑菌活性成分提取工艺研究

姚志蕊, 曹光群, 杨成   

  1. 1. 江南大学 化学与材料工程学院, 江苏, 无锡, 214122
  • 收稿日期:2008-03-10 修回日期:1900-01-01 出版日期:2008-10-30 发布日期:2008-10-30
  • 通讯作者: 曹光群,男,江苏宜兴人,高级工程师,硕士生导师,主要从事日用化学品的研究;E-mail:gqcao@jiangnan.edu.cn。

Research on Extraction of Anti-microbial Active Components from Phyllostachys pubescens Mazel ex H. de Lehaie Leaves

YAO Zhi-rui, CAO Guang-qun, YANG Cheng   

  1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
  • Received:2008-03-10 Revised:1900-01-01 Online:2008-10-30 Published:2008-10-30

摘要: 研究了毛竹叶活性成分的提取工艺及其抑菌效果.用液体培养基稀释法对金黄色葡萄球菌和大肠杆菌进行了最低抑菌浓度(MIC)测定.结果表明,温度60℃、乙醇体积分数60%、液固比20:1 (mL:g)、提取2h条件下毛竹叶提取物对金黄色葡萄球菌和大肠杆菌有很强的抑制作用,对金黄色葡萄球菌的MIC为3.125g/L,对大肠杆菌的MIC为6.25g/L.

关键词: 毛竹叶, 提取, 抑菌活性, 最低抑菌浓度

Abstract: Extraction of active components from moso bamboo (Phyllostachys pubescens Mazel ex H. de Lehaie) leaves and their anti-microbial actives were studied. The minimum inhibitory concentration (MIC) of the active components from P. pubescens leaves against Staphylococcus aureus and Escherichia coli was determined by serial liquid dilution method. The results showed that anti-microbial effect of extracts from P. pubescens leaves against S. aureus and E. coli was optimum with the extraction condition: 60℃, 60% (volume fraction)ethanol, ratio of liquid to solid 20:1, 2h. The MIC of the extracts from P. pubescens leaves was 3.125g/L against S. aureus, and 6.25g/L against E. coli.

Key words: Phyllostachys pubescens Mazel ex H. de Lehaie leaves, extraction, anti-microbial activity, minimum inhibitory concentration

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