黄花蒿中青蒿素的柱前衍生HPLC-UV分析

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (3): 115-119.

黄花蒿中青蒿素的柱前衍生HPLC-UV分析

张荣沭^1,3, 王炎², 赵敏¹

1. 东北林业大学, 生命科学学院, 黑龙江哈尔滨 150040;2. 哈尔滨工业大学理学中心, 黑龙江哈尔滨 150001;3. 齐齐哈尔医学院附属第二医院, 黑龙江齐齐哈尔 161006

收稿日期:2008-06-01 修回日期:1900-01-01 出版日期:2009-06-30 发布日期:2009-06-30
通讯作者: 赵敏,教授,博士生导师,研究领域:微生物制药。

Analysis of Artemisinin from Artemisia annua L. by HPLC-UV Using Pre-column Derivatization

ZHANG Rong-shu^1,3, WANG Yan², ZHAO Min¹

1. Life Science College of Northeast Forestry University, Harbin 150040, China;2. Science Research Center, Harbin Institute of Technology, Harbin 150001, China;3. Second Clinical Medical Institute of Qiqihar Medical College, Qiqihar 161006, China

Received:2008-06-01 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30

摘要/Abstract

摘要： 研究衍生化条件对HPLC-UV法测定黄花蒿青蒿素含量的影响。建立了黄花蒿青蒿素的柱前衍生HPLC-UV测定方法:即青蒿素标准品溶液(或黄花蒿提取浸膏)以体积分数为 95% 乙醇补充(溶解)至15.0mL,再加入质量分数为 0.2% 的NaOH 8.0mL,45℃ 水浴 30min后冷却至室温,加入 0.08mol/L 的HAc 10mL 摇匀,以 95% 乙醇定容至 50mL 容量瓶中;HPLC分离,260 nm 紫外检测。所得方法检测线性范围为0～200mg/L,相对标准偏差(RSD)值为 1.40%,平均回收率(n=5)为 100.1%。用该方法测定了黑龙江引种的黄花蒿提取物样品,稳定性RSD值为 4.35%,重现性RSD值为 4.96%。证明该方法简单、稳定、重现性好。

关键词: 青蒿素, 黄花蒿, 高效液相色谱

Abstract: Effect of pre-column derivatization on determination of artemisinin from Artemisia annua by HPLC-UV was studied. The modified determinatin method of HPLC-UV with pre-column derivatization was established as follows:the standard solution of artemisinin (or its extract) was supplementally added to 15.0mL with 95% ethanol, then added with 0.2% NaOH 8.0mL, andheated in a water bath at 45℃ for 30min. After it was cooled to room temperature, acidified with 0.08mol/L HAc 10mL and shaked. Then it was filled to marked volume of 50 mL with 95% ethanol, separated by HPLC, and detected at 260nm. The methodgave a wide range of linearity in the concentration range from 0 to 200mg/L. The relative standard deviation(RSD) was 1.40% and average recovery(n=5) was 100.1%. The method was used to determine artemisinin extracted from introduced sample. The result demonstrated that the RSD of stabilization and reproducibility were 4.35% and 4.96%, respectively. It fully proved that the method is simple, stable and reproducible.

Key words: artemisinin, Artemisia annua L., HPLC

中图分类号:

TQ351.0

张荣沭;王炎;赵敏. 黄花蒿中青蒿素的柱前衍生HPLC-UV分析[J]. 林产化学与工业, 2009, 29(3): 115-119.

ZHANG Rong-shu;WANG Yan;ZHAO Min. Analysis of Artemisinin from Artemisia annua L. by HPLC-UV Using Pre-column Derivatization[J]. , 2009, 29(3): 115-119.

[1]	倪林, 邱亚铁, 李艳蕾, 邹小兴, 徐会有. HPLC法同时测定闽产圆齿野鸦椿中两种色原酮碳苷的含量[J]. 林产化学与工业, 2018, 38(4): 103-108.
[2]	徐杰, 程传可, 胡国元, 杨春雷, 余君, 杨锦鹏. 臭蒿抗菌成分的分离和鉴定[J]. 林产化学与工业, 2018, 38(2): 80-86.
[3]	刘玉楠, 张朝晖, 黄小花, 饶维. 姜黄素磁性印迹固相萃取材料的制备及其应用研究[J]. 林产化学与工业, 2014, 34(6): 37-43.
[4]	邢雅丽, 毕良武, 赵振东, 韩路路, 夏田娟. 中心复合设计法优化熊果酸和齐墩果酸的HPLC测试条件[J]. 林产化学与工业, 2014, 34(4): 37-41.
[5]	杨艳;贺丽苹;高向阳. 檀香叶中的γ-氨基丁酸的HPLC测定研究[J]. 林产化学与工业, 2013, 33(2): 134-138.
[6]	肖卓炳;郭满满;郭瑞轲;田春莲. 热分析法研究青蒿素的纯度、热分解动力学及贮存期[J]. 林产化学与工业, 2013, 33(1): 13-20.
[7]	骆沙曼;丁为民;于涛;王洋. 桦木醇的液泛提取工艺研究[J]. 林产化学与工业, 2013, 33(1): 54-58.
[8]	刘建群;任晓静;舒积成;张锐;张维;潘景行. HPLC法同时测定凤尾草中5种黄酮类成分的含量[J]. 林产化学与工业, 2013, 33(1): 117-120.
[9]	林霄;董晓敏;陈明生;黄艳;刘布鸣;. 高效液相色谱法同时测定互叶白千层中的槲皮素和山奈酚[J]. 林产化学与工业, 2012, 32(4): 113-116.
[10]	叶建中;王成章;陈虹霞;周昊. 油橄榄叶中羟基酪醇的含量变化规律研究[J]. 林产化学与工业, 2011, 31(2): 69-74.
[11]	陈虹霞;王成章;叶建中;周昊. 油茶饼粕中黄酮苷类化合物的分离与结构鉴定[J]. 林产化学与工业, 2011, 31(1): 13-16.
[12]	姜贵全;高文韬;李亚荣;庞久寅. HPLC法测定吉林地区三种乌头双酯型生物碱含量[J]. 林产化学与工业, 2010, 30(4): 85-88.
[13]	丁为民;周丹;于涛;黄靖姝;阎秀峰;王洋. 均匀设计法优化桦木醇超声波辅助提取工艺[J]. 林产化学与工业, 2010, 30(3): 78-82.
[14]	焦志敏;陈龙胜;许舒雯;陈兰;任凤莲. HPLC测定油橄榄不同部位山楂酸的含量[J]. 林产化学与工业, 2010, 30(2): 23-26.
[15]	徐元君;罗丽萍;丽艳;陈滨;付宇新;高荫榆. HPLC测定两种中国蜂胶醇提物化学组成[J]. 林产化学与工业, 2010, 30(2): 61-66.