关键词: 栀子, 愈伤组织, 环烯醚萜苷

Abstract: To promote the development of normalized cultivation of Gardenia jasminoides Ellis, the rule of iridoidglycosides synthesis in G. jasminoides callus was studied by plant cell culture technology,HPLC and UV. Thegrowth of G.jasminoides callus and the accumulation of iridoidglycosides are not proceeded synchronically, callusgrows first and then its secondary metabolic products are synthesized. Sucrosehas obvious effect to regulate the synthesis of iridoidglycosides.When concentration of sucrose is between 3% and 8%, thegrowth of callus and synthesis of iridoidglycosides aregreatly increased. The addition of organics such as beef cream and peptone in culture medium could promote callusgrowth, but inhibited the accumulation of iridoidglycosides. Callusgrowth could be induced when concentrations of Fe²⁺ and Mn²⁺ were properly increased in Murashige & Skoog(MS) culture medium. Mn²⁺ could increase accumulation of iridoidglycosides, but Fe²⁺had no obvious effect on accumulation of iridoidglycosides.Thegrowth of G. jasminoides callus and accumulation of iridoidglycosides were suppressed when ammonium salt or nitrate was used alone in culture medium as nitrogen source. Under the condition of c(NH₄⁺):c(NO₃^-) 1:1 and total nitrogen source 60 mmol/L, both total biomass and amount of iridoidglycosides arrived to the maximum. Increase of KH₂PO₄ in culture medium between 0 and 0.5 mmol/L couldgreatly increase callusgrowth which arrived to the maximum at 1.5 mmol/L.

Key words: Gardenia jasminoides Ellis, callus, iridoid glycosides