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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (1): 63-67.

• 研究报告 • 上一篇    下一篇

油橄榄叶中羟基酪醇含量及提取工艺研究

叶建中1,2, 王成章1,2, 陈虹霞1, 董艳鹤1   

  1. 1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局 林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏 南京 210042;2. 中国林业科学研究院 林业新技术研究所, 北京 100091
  • 收稿日期:2010-10-11 修回日期:1900-01-01 出版日期:2011-02-28 发布日期:2011-02-28
  • 通讯作者: 王成章,湖北汉川人,研究员,主要从事天然产物研究与利用;E-mail:wangczlhs@sina.com。

Content Determination and Extraction Technology of Hydroxytyrosol from Olive Leaves

YE Jian-zhong1,2, WANG Cheng-zhang1,2, CHEN Hong-xia1, DONG Yan-he1   

  1. 1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.on Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;2. Institute of New Technology of Forestry, CAF, Beijing 100091, China
  • Received:2010-10-11 Revised:1900-01-01 Online:2011-02-28 Published:2011-02-28

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摘要： 油橄榄含有橄榄苦苷、黄酮类和羟基酪醇等多酚抗氧化物。采用ODS(Φ4.6mm×200mm,5μm)色谱柱,紫外检测波长为230nm, 甲醇-0.2%醋酸水溶液6:94(体积比)为流动相,流速1mL/min,建立羟基酪醇的HPLC分析方法,对不同品种的油橄榄叶进行含量分析。结果表明:10个品种油橄榄叶中羟基酪醇质量分数范围在0.3%～0.8%之间,小莱星含量最高,配多灵含量最低。通过单因素和正交试验研究了超声波提取油橄榄叶中羟基酪醇的工艺,1g油橄榄叶粉末正交试验的优化结果为:功率120W、90%甲醇提取、料液比1:20(g:mL)、时间20min。采用90%甲醇提取油橄榄叶,提取液经AB-8大孔树脂、硅胶柱层析和HPLC制备色谱,分离质量分数95%以上的单体,通过MS、NMR鉴定该化合物为羟基酪醇。

关键词: 油橄榄叶, 羟基酪醇, 正交试验, 化学结构

Abstract: Many polyphenolic, antioxidants such as oleuropein, flavonoids and hydroxytyrosol exist in olive leaves. The identification of hydroxytyrosol in different breeds of olive leaves was carried out by HPLC moritored at 230nm with ODS column(Φ4.6mm×200mm,5μm), MeOH-0.2% acetic acid buffer 6:94(volume ratio)as mobile phase at flow rate 1mL/min.The contents of hydroxytyrosol were distributed from 0.3% to 0.8% in 10 breeds. The ultrasonic extract of hydroxytyrosol was studied by single factor and orthogonal experiment.The optimum extraction conditions were as follows:ultrasonic power 120W, 90% MeOH-H2O as extractant, material-liquid ratio 1:20(g:mL), extraction time 20min.Meanwhile, a single compound of 95% content was seperated by AB-8 macroporous resin, silica gel column chromatography and preparative HPLC. It was identificated to be hydroxytyrosol by MS and NMR.

Key words: olive leaves, hydroxytyrosol, orthogonal test, chemical structure

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