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林产化学与工业

林产化学与工业 ›› 2012, Vol. 32 ›› Issue (6): 47-51.

• 1研究报告 • 上一篇    下一篇

HPLC-MS法表征坝漆酚类化合物的结构

何源峰1, 王成章1,2, 陆榕3   

  1. 1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏 南京 210042;2. 中国林业科学研究院 林业新技术研究所, 北京 100091;3. 明治大学, 东京 日本 214-8571
  • 收稿日期:2012-04-15 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
  • 通讯作者: 王成章,研究员,博士生导师,研究领域为天然产物研究与应用;E-mail:wangczlhs@sina.com。

Identification of Chemical Structure of Urushiol from Maoba Lacquer by HPLC-MS

HE Yuan-feng1, WANG Cheng-zhang1,2, LU Rong3   

  1. 1. Institute of Chemical Industry of Forestry Products,CAF;Nation Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering,SFA;Key Lab.of Biomass Energy and Material,Jiangsu Province, Nanjing 210042, China;2. Institute of New Technology of Forestry,CAF, Beijing 100091, China;3. Meiji University, Tokyo 214-8571, Japan
  • Received:2012-04-15 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

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摘要： 应用中低压柱分离毛坝漆酚类化合物,漆酚质量分数90%以上。应用HPLC、HPLC-MS、MS分析表征毛坝漆漆酚化学结构,HPLC以甲醇-水体积比9∶1为流动相, 总流速1mL/min,UV 210nm能够分离18个酚类化合物,HPLC-MS表征13个酚类化合物,主要为C15不饱和三烯漆酚和C17不同饱和度的漆酚。漆酚的中低压柱分离及HPLC跟踪为漆酚提供了快速分离方法。

关键词: 毛坝漆, 漆酚, HPLC-MS, 结构分析

Abstract: Middle pressure chromatography was used to purify urushiol. Its purity reached above 90%. HPLC,HPLC-MS,MS was applied to analyze urushiol that was extracted from Maoba lacquer. HPLC was performed under a methanol-water system(volume ratio) with a flow rate of 1mL/min. Eighteen peaks appeared under UV detector of 210 nm. Thirteen compounds were separated by HPLC-MS, mainly contained C15/17 with different degrees of unsaturation. The middle pressure chromatography and HPLC could be used as a efficient preparation and analysis method.

Key words: Maoba lacquer, urushiol, HPLC-MS, structure analysis

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