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林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (5): 97-102.

• 研究报告 • 上一篇    下一篇

糖蜜酒精废液色素的提取分离及其抗氧化活性的研究

王标诗1, 李汴生1, 曾庆孝1, 刘慧霞2   

  1. 1. 华南理工大学 轻工与食品学院, 广东 广州 510640;2. 广西大学轻工与食品工程学院, 广西 南宁 530004
  • 收稿日期:2007-01-12 修回日期:1900-01-01 出版日期:2007-10-30 发布日期:2007-10-30

Extraction of Pigment from Molasses Alcohol Wastewater and Its Antioxidant Activity

WANG Biao-shi1, LI Bian-sheng1, ZENG Qing-xiao1, LIU Hui-xia2   

  1. 1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China;2. College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China
  • Received:2007-01-12 Revised:1900-01-01 Online:2007-10-30 Published:2007-10-30

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摘要： 采用大孔树脂提取分离糖蜜酒精废液色素并研究其抗氧化活性.通过对5种不同型号的大孔树脂进行色素吸附试验,选出吸附效果较好的树脂型号HPD-00和D301-R,用酒精溶液进行洗脱得出40%的酒精为较好的洗脱剂.再通过3种不同的体系:亚硝酸盐、超氧阴离子(O2-)和1,1-二苯基苦基苯肼(DPPH)体系研究酒精废液色素的抗氧化活性.结果表明,酒精废液色素在亚硝酸盐体系和DPPH体系中的抗氧化活性较好,且随质量浓度的增加抗氧化效果越强.在亚硝酸盐体系中,色素对亚硝酸盐清除率的半抑制质量浓度(IC50)约为0.75g/L;在DPPH体系中,色素对DPPH·清除率的IC50约为0.07g/L.

关键词: 糖蜜酒精废液, 色素, 抗氧化活性

Abstract: The extraction of pigment from molasses alcohol wastewater and its antioxidant activity were studied. The macroporous resins HPD-600 and D301-R having better effect were selected through adsorption experiments using 5 macroporous resins on the pigment. The antioxidant activity from pigment from alcohol wastewater was studied in three different systems (NO2-, O2- and DPPH·). The results showed that their antioxidant activities were better in the NO2- and DPPH· system than in the O2- system and their antioxidant activities were enhanced with the increasing of concentration. In nitrite system, the half-inhibition concentraction (IC50) of the pigment toward nitrite was about 0.75 g/L. In DPPH·system, the IC50 of the pigment toward DPPH· was about 0.07 g/L.

Key words: molasses alcohol wastewater, pigment, antioxidant activity

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