红叶李叶化学成分及其体外抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2015.05.019

摘要/Abstract

摘要： 研究红叶李叶的化学成分和抗氧化活性。以硅胶、葡聚糖凝胶LH-20、高效制备液相色谱等分离红叶李叶乙醇提取物,根据光谱数据结合理化性质鉴定17个化合物,分别为β-谷甾醇(1)、豆甾醇(2)、胡萝卜苷(3)、羽扇豆醇 (4)、熊果酸(5)、阿江榄仁酸(6)、Niga-ichigoside F1(7)、芹菜素 (8)、山奈酚(9)、山柰酚-3-O-β-D-葡萄糖苷(10)、槲皮素(11)、槲皮素4'-O-β-D-葡萄糖苷(12)、金丝桃苷(13)、木犀草素(14)、木犀草素-4'-O-β-D-葡萄糖苷(15)、芦丁(16)、没食子酸(17)。所有化合物均为首次从红叶李叶中分离得到。以DPPH自由基清除能力检测所得化合物的抗氧化能力,结果显示,化合物11, 14和17表现出显著的自由基清除能力,IC₅₀分别为32.18、34.65和34.12 mmol/L,活性强于Vc(IC₅₀为55.22 mmol/L)。

关键词: 红叶李叶, 化学成分, 抗氧化活性

Abstract: Ethanol extracts from the leaves of Prunus cerasifera Ehrh.cv.Atropurpurea Jacg. were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including ¹H, ¹³C NMR. Seventeen compounds were isolated and identified as β-sitosterol (1), stigmasterol (2), daucosterol (3), lupeol (4), ursolic acid (5), arjunolic acid (6), Niga-ichigoside F1 (7), apigenin (8), kaempferol (9), kaempferol-3-O-β-D-glucoside (10), quercetin (11), quercetin-4'-O-β-D- glucoside (12), hyperin (13), luteolin (14), luteolin-4'-O-β-D-glucoside (15), rutin (16), gallic acid (17). All compounds were isolated from the leaf for the first time. Compounds 11, 14 and 17 showed stronger antioxidant activities by DPPH scavenging activity with IC₅₀ inhibition rates of 32.18, 34.65 and 34.12 mmol/L, respectively, compared with the Vc IC₅₀ inhibition rate of 55.22 mmol/L.

Key words: Prunus cerasifera Ehrh.cv.Atropurpurea Jacg., chemical constituents, antioxidant activities

中图分类号:

TQ35

卫强, 纪小影, 龙先顺, 李前荣, 尹浩. 红叶李叶化学成分及其体外抗氧化活性研究[J]. 林产化学与工业, 2015, 35(5): 116-122.

WEI Qiang, JI Xiao-ying, LONG Xian-shun, LI Qian-rong, YIN Hao. Chemical Constituents Extracted from Leaves of Prunus cerasifera Ehrh.cv. Atropurpurea Jacg. and Their Antioxidant Activities in vitro[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 116-122.

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