金雀花提取物正丁醇沉淀部分化学成分研究

doi:10.3969/j.issn.0253-2417.2015.03.021

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (3): 121-124.doi: 10.3969/j.issn.0253-2417.2015.03.021

金雀花提取物正丁醇沉淀部分化学成分研究

张妮¹, 穆淑珍², 王家胜¹, 孔琪¹, 余正文¹

1. 贵州师范大学生命科学学院, 贵州贵阳 550001;
2. 贵州省中国科学院天然产物化学重点实验室, 贵州贵阳 550002

收稿日期:2014-03-07 出版日期:2015-06-25 发布日期:2015-07-03
通讯作者: 余正文(1973—),教授,硕士生导师,主要从事天然药物化学专业的研究;E-mail:yuzhengwen2001@126.com。 E-mail:yuzhengwen2001@126.com
作者简介:张妮(1988—),女,陕西咸阳人,硕士生,主要从事药用资源的开发与利用研究工作
基金资助:
贵阳市现代药业计划筑科合同(2012204)

Chemical Constituents of n-Butanol-insoluble Portion from Parochetus communis Buch.Extract

ZHANG Ni¹, MU Shu-zhen², WANG Jia-sheng¹, KONG Qi¹, YU Zheng-wen¹

1. School of Life Science, Guizhou Normal University, Guiyang 550001, China;
2. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China

Received:2014-03-07 Online:2015-06-25 Published:2015-07-03

摘要/Abstract

摘要： 采用乙酰化方法对金雀花中正丁醇沉淀部分的化学成分进行研究。金雀花的地上部分用75%的乙醇提取,乙醇总浸膏用去离子水饱和悬浮,依次用石油醚、乙酸乙酯、正丁醇萃取;其中正丁醇萃取物沉淀部分乙酰化衍生后采用各种柱色谱进行分离纯化,结合理化性质及光谱数据鉴定化合物结构。从正丁醇沉淀部分分离出4个化合物,分别鉴定为β-谷甾醇、1-三十烷醇、美迪紫檀素及车轴草醇,所有的化合物均为首次从该植物中分离得到。此外,乙酰化反应使得正丁醇沉淀部分的溶解性增加,极性降低,为其他植物中大极性化合物的分离纯化提供参考。

关键词: 金雀花, 乙酰化方法, 正丁醇沉淀, 化学成分

Abstract: The chemical constituents of n-butanol-insoluble portion of Parochetus communis Buch extract were studied by acetylation.The aerial part of P.communis was extracted by 75% ethanol and the ethanol extract was suspended in distilled water and successively extracted by petroleum ether,ethyl acetate,and n-butanol.The insoluble portion of n-butanol extract was partly acetylated,then various column chromatographic methods were used for the isolation and purification.The chemical structures of compounds were elucidated on the basis of physicochemical properties and spectral date.Six compounds were isolated from n-butanol-insoluble portion of P.communis Buch and identified as β-sitosterol,1-triacontanol,medicarpin and pratol.All compounds were isolated from the medical plant for the first time.In addition,the solubility of n-butanol-insoluble portion was increased and the polarity of the chemical composition was decreased by acetylation.This method provides a new reference for the separation of high-polar compounds in other plant.

Key words: Parochetus communis Buch., acetylation, n-butanol-insoluble portion, chemical constituents

中图分类号:

TQ35

张妮, 穆淑珍, 王家胜, 孔琪, 余正文. 金雀花提取物正丁醇沉淀部分化学成分研究[J]. 林产化学与工业, 2015, 35(3): 121-124.

ZHANG Ni, MU Shu-zhen, WANG Jia-sheng, KONG Qi, YU Zheng-wen. Chemical Constituents of n-Butanol-insoluble Portion from Parochetus communis Buch.Extract[J]. Chemistry and Industry of Forest Products, 2015, 35(3): 121-124.

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金雀花提取物正丁醇沉淀部分化学成分研究

Chemical Constituents of n-Butanol-insoluble Portion from Parochetus communis Buch.Extract

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