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林产化学与工业 ›› 2013, Vol. 33 ›› Issue (1): 91-96.doi: 10.3969/j.issn.0253-2417.2013.01.017

• 1研究报告 • 上一篇    下一篇

鼠掌老鹳草抗氧化活性成分季节变化研究

杨雨春1, 李吉2,3, 付玉杰2,3, 祖元刚2,3   

  1. 1. 吉林省林业科学研究院, 吉林 长春 130033;2. 森林植被生态学教育部重点实验室, 黑龙江 哈尔滨 150040;3. 林业生物制剂工程中心, 黑龙江 哈尔滨 150040
  • 收稿日期:2011-12-20 修回日期:1900-01-01 出版日期:2013-02-28 发布日期:2013-02-28
  • 通讯作者: 付玉杰,教授,博士,研究领域为天然产物开发;E-mail:yujie_fu2002@yahoo.com。

Seasonal Variation of Antioxidant Components of Geranium sibiricum Linne

YANG Yu-chun1, LI Ji2,3, FU Yu-jie2,3, ZU Yuan-gang2,3   

  1. 1. Forstry Academy of Jilin Province, Changchun 130033, China;2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Harbin 150040, China;3. Engineering Research Center of Forest was higher in the summer and presented highest Bio-preparation, Ministry of Education, Harbin 150040, China
  • Received:2011-12-20 Revised:1900-01-01 Online:2013-02-28 Published:2013-02-28

摘要: 研究了鼠掌老鹳草全草、根、茎和叶片的生物量以及不同部位中柯里拉京、老鹳草素和总酚含量的季节变化,并通过抗氧化活性指导确定了鼠掌老鹳草的采收部位、采收时间。结果表明:1 g鼠掌老鹳草全草中的多酚类成分在营养生长期含量较高(137.567 3±1.890 7)mg/g(以没食子酸(GAE)质量表示,对提取物而言,下同),生殖生长期和枯萎期含量迅速降低((74.751 8±1.010 1)mg/g),而总黄酮含量在夏季较高,在枯萎期叶含量最大。鼠掌老鹳草的最佳采收时间和采收部位分别是7月份和全草,其总体抗氧化活性(FRAP)值为(12.321 5±0.188 2)mmol/g 。PCA试验表明总酚含量、柯里拉京、老鹳草素之间具有较好的相关性(r>0.753),而且它们与抗氧化活性之间的相关性也较好(|r|>0.75)。

关键词: 鼠掌老鹳草, 多酚类成分, 季节变化, 抗氧化活性

Abstract: Seasonal variation of corilagin, geraniin as well as total phenolic contents during the growth period, where were obtained from the whole grass, roots, stems and leaves of Geranium sibiricum Linne, were investigated. The antioxidant capacity (FRAP, DPPH, ABTS) were evaluated to confirm the optimum picking part and stage. Results showed that corilagin (CG), geraniin (GE) and total phenolic contents (TPC) increased dramatically in the vegetative growth period (137.567 3±1.890 7 GAE/g extracts)and decreased gradually during the reproductive growth and withering period (74.7518±1.010 1 GAE/g extracts), while the total flavonoid contents (TF) was high in summer and reached the highest during the withering period. The optimum picking part and time were the whole grass and July, respectively, and the total antioxidant capacity (FRAP) was (12.321 5±0.188 2)mmol/g extracts. Correlation of the data obtained from principal composition analysis (PCA) showed that TPC, CG and GE had high relationship (r>0.753) and they also had high relationship with antioxidant capacity (|r|>0.75).

Key words: G.sibiricum Linne, polyphenol, seasonal variation, antioxidant capacity

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