欢迎访问《林产化学与工业》,

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (1): 103-106.

• 研究报告 • 上一篇    下一篇

Klebsiella oxytoca发酵木糖生产2,3-丁二醇的研究

冯燕青, 夏黎明   

  1. 浙江大学 化学工程与生物工程系, 浙江 杭州 310027
  • 收稿日期:2008-01-05 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28
  • 通讯作者: 夏黎明,博士生导师,主要从事可再生资源利用方面的研究。

Production of 2,3-Butanediol from Fermentation of Xylose by Klebsiella oxytoca ZU-03

FENG Yan-qing, XIA Li-ming   

  1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
  • Received:2008-01-05 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

摘要: 利用一株克雷伯氏菌(Klebsiella oxytocaZU-03)发酵木糖生产2,3-丁二醇,并对主要发酵工艺参数进行了优化。实验结果表明:在木糖质量浓度90g/L、玉米浆质量浓度6g/L、接种量10%(体积分数)、初始pH值5.5、30℃的条件下发酵96h,木糖利用率为89%,2,3-丁二醇的质量浓度为36.22g/L,得率为0.45g/g,达到了理论值的90%。

关键词: 3-丁二醇, 木糖, 半纤维素, 发酵

Abstract: The production of 2,3-butanediol was carried out from fermentation of xylose by Klebsiella oxytoca ZU-03, in which xylose was the main reducing sugar in hemicellulosic hydrolysate. Under optimized conditions: xylose mass concentration 90g/L, corn liquor mass concentration 6g/L, inoculum 10% (volume fraction), initial pH value 5.5 and 30℃, concentration of 2,3-butanediol reached 36.22g/L and yield of 2,3-butanediol from xylose was 0.45g/g.

Key words: 3-butanediol, xylose, hemicellulose, fermentation

中图分类号: