生物预处理对油樟精油组成、油细胞和内生菌群的影响

doi:10.3969/j.issn.0253-2417.2021.05.008

Abstract

Abstract:

The leaves of Cinnamomum longepaniculatum(Gamble) N.Chao ex H. W. Li. were pretreated by adding water, yeast and complex bacteria respectively, and the essential oil of C. longepaniculatum leaves was extracted after pretreatment. The GC-MS was used to characterize the chemical composition of essential oil. The tissue transparency method was used to observe the morphology of the oil cells after pretreatment, and calculate the density of oil cells; finally, through gene sequencing, characterize the endophytic community structure of C. longepaniculatum leaves and analyze the correlation between the endophytic bacteria community and biological pretreatment. The results showed that yeast pretreatment and complex bacteria pretreatment increased the extraction yield of essential oil by 33.6% and 49.8%, respectively, and slightly reduced the relative content of 1, 8-cineole, the minor components such as α-terpineol, β-pinene and γ-terpinene were slightly increased; the oil cell density of C.longepaniculatum leaves after pretreatment with water, yeast, and complex bacteria were (37±2), (27±2) and (25±5) per 1 mm², which were less than that of fresh leaves, (37±3) per 1 mm²; Based on distance-based redundancy analysis(dbRDA) and correlation heatmap analysis, it is found that pretreatment methods havd a greater impact on the endophytic community structure of camphor leaves. γ-Proteobacteria, α-proteobacteria, cyanobacteria and acidobacteria were the most affected species in class level. The order of the degree of influence was complex bacteria pretreatment>yeast pretreatment=water pretreatment. The addition of complex bacteria was significantly related to a variety of endophytes, especially the abundance of planktomycetes, acid bacilli and bacillus.

Key words: Cinnamomum longepaniculatum, essential oil, oil cells, endophytic microbe

CLC Number:

TQ35

Xian CHENG, Liangwu BI, Shengnan LI, Yuxiang CHEN, Zhendong ZHAO, Kailin MO. Effects of Biological Pretreatment on Constituents, Oil Cells Morphology and Endophytic Microbial Communities of Cinnamomum longepaniculatum[J]. Chemistry and Industry of Forest Products, 2021, 41(5): 51-57.

Figures/Tables 6

Table 1

Table 2

Fig.1

Table 3

Fig.2

Fig.3

References 18

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预处理方式 pretreatment methods	水 water	酵母菌 yeast	复合菌 complex bacteria
无blank(B)
水water (W)	1
酵母菌yeast(Y)	1	1
复合菌complex bacteria (C)	1	1	1

序号 No.	t_R/min	化合物 compound	分子式 fomula	相对峰面积relative area¹⁾/%
序号 No.	t_R/min	化合物 compound	分子式 fomula	空白 blank	水预处理 water	酵母菌预处理 yeast	复合菌预处理 complex bacteria
1	6.955	α-蒎烯α-pinenne	C₁₀H₁₆	0.31	0.42	0.29	0.50
2	7.217	β-蒎烯β-pinenne	C₁₀H₁₆	2.23	2.57	3.44	3.94
3	9.460	β-水芹烯β-phellandrene	C₁₀H₁₆	5.36	5.83	4.47	7.13
4	9.530	莰烯camphene	C₁₀H₁₆	0.05	0.15	0.44	0.28
5	12.623	邻伞花烃o-cymene	C₁₀H₁₄	0.29	0.18	0.27	0.49
6	13.415	对伞花烃p-cymene	C₁₀H₁₄	0.33	0.58	0.73	0.51
7	13.887	1, 8-桉叶素1, 8-cineole	C₁₀H₁₈O	57.25	53.89	54.58	51.56
8	19.995	罗勒烯ocimene	C₁₀H₁₆	0.05	0.37	0.36	0.78
9	31.724	侧栢烯thujene	C₁₀H₁₆	0.30	0.15	0.68	0.50
10	32.865	4-松油醇4-terpineol	C₁₀H₁₈O	4.99	3.87	2.11	2.80
11	33.945	α-松油醇α-terpineol	C₁₀H₁₈O	24.91	24.63	25.58	26.04
12	35.413	α-水芹烯α-phellandrene	C₁₀H₁₆	0.23	0	0.19	0
13	36.075	γ-松油烯γ-terpinene	C₁₀H₁₆	2.30	4.33	4.16	3.78
14	36.454	石竹烯caryophyllene	C₁₅H₂₄	0.79	1.08	0.89	0.83
15	36.659	甘香烯elixene	C₁₅H₂₄	0.37	0.71	0.22	0.43
16	36.887	大根香叶烯D germacrene D	C₁₅H₂₄	0.24	1.24	1.59	0.43

内生菌 endophyte	纲水平上内生菌相对丰度/% percent of community abundance on class level
内生菌 endophyte	空白 blank	水 water	酵母菌 yeast	复合菌 complex bacteria
γ-变形菌γ-proteobacteria	79.84	44.51	64.11	16
α-变形菌α-proteobacteria	1.32	3.13	6.04	42.52
放线菌actinobacteria	15.22	26.74	22.94	7.35
蓝细菌cyanobacteria	0	0	0	17.88
杆菌bacilli	2.64	22.74	4.12	1
酸杆菌acidobacteria	0.11	0.03	0.29	9.99
拟杆菌bacteroidia	0.51	2.16	1.6	0.88
嗜热油菌thermoleophilia	0.06	0.02	0.08	1.54
浮霉菌planctomycetes	0	0	0	1.39
其他others	0.3	0.69	0.83	1.44

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Effects of Biological Pretreatment on Constituents, Oil Cells Morphology and Endophytic Microbial Communities of Cinnamomum longepaniculatum

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