Chemistry and Industry of Forest Products ›› 2022, Vol. 42 ›› Issue (1): 10-20.doi: 10.3969/j.issn.0253-2417.2022.01.002
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Hongxia CHEN1(), Hao ZHOU1,2, Ran TAO1, Zhiwen QI1, Wenjun LI1, Chengzhang WANG1,2,*()
Received:
2021-01-04
Online:
2022-02-28
Published:
2022-03-11
Contact:
Chengzhang WANG
E-mail:shirenyahui@126.com;wangczlhs@sina.com
CLC Number:
Hongxia CHEN, Hao ZHOU, Ran TAO, Zhiwen QI, Wenjun LI, Chengzhang WANG. Study on Polyphenols in Lacquer Tree by Matrix Solid-phase Dispersion and HPLC-IT-TOF/MS[J]. Chemistry and Industry of Forest Products, 2022, 42(1): 10-20.
Table 1
HPLC-IT-TOF/MS analysis of Shaanxi lacquer tree extract"
峰号 No. | t/min | 准分子离子峰 quasi-molecular ion peak | 负离子模式下碎片峰 fragment peak in negative ion mode | 分子式 molecular formula | 鉴定结果 appraisal result |
1 | 1.133 | 169.0173[M-H]- | 125.0277 | C7H6O5 | 没食子酸 gallic acid |
2 | 3.187 | 183.0321[M-H]- | 125.0192 | C8H8O5 | 没食子酸甲酯 methyl gallate |
3 | 6.200 | 197.0482[M-H]- | 169.0171, 124.9759 | C9H10O5 | 没食子酸乙酯 ethyl gallate |
4 | 7.400 | 287.0564[M-H]- 289.0703[M+H]+ | 269.0497, 254.9302, 226.9803, 150.9935 | C15H12O6 | 黄颜木素 fustin |
5 | 9.587 | 303.0521[M-H]- 305.0668[M+H]+ | 275.0532, 271.0604, 242.9193, 179.0254, 150.9933 | C15H12O7 | 花旗松素 taxifolin |
6 | 10.760 | 271.0631[M-H]- 273.0755[M+H]+ | 243.0669, 238.9312, 226.9801, 162.9949 | C15H12O5 | 3, 4′, 7-三羟基黄烷酮 garbanzol |
7 | 11.933 | 315.0512[M-H]- 317.0651[M+H]+ | 301.0479, 255.0420, 178.9299, 150.9933 | C16H12O7 | 异鼠李素 isorhamnetin |
8 | 13.453 | 447.0941[M-H]- 449.1070[M+H]+ | 300.0273, 271.2206, 255.0471, 242.9359 | C21H20O11 | 槲皮苷 quercitrin |
9 | 14.720 | 285.0405[M-H]- 287.0552[M+H]+ | 257.0923, 228.9805, 162.9942 | C15H10O6 | 漆黄素 fisetin |
10 | 17.920 | 269.0454[M-H]- 271.0597[M+H]+ | 240.9241, 222.9809, 136.9943 | C15H10O5 | 硫黄菊素 sulfuretin |
Table 2
Comparison of MSPD method with other methods"
提取方法 extraction method | 分析方法 analysis method | 分析物1) analyte | 样品量/g sample dose | 溶剂solvent | 条件condition | 参考文献 reference | |||
种类 type | 用量 dose/mL | 时间/min time | 温度/℃ temp. | ||||||
热回流提取 heat reflux extraction | UV | 总多酚 total polyphenols | 1 | 水 water | 25 | 555 | 89.95 | [ | |
热回流提取 heat reflux extraction | LC-MS/MS | 主要化学成分 main chemical | 3 | 60%乙醇水溶液alcohol solution | 90 | 600 | 85 | [ | |
热回流提取 heat reflux extraction | LC-MS/MS | 主要化学成分 main chemical | 100 | 水water | 1000 | 180 | 100 | [ | |
负压提取 vacuum extraction | UV | 总多酚 total polyphenols | 5 | 83%乙醇水溶液 alcohol solution | 100 | 90 | — | [ | |
MSPD | UV+ HPLC-IT-TOF/MS | 总多酚,主要化学成分 total polyphenols, main chemical | 0.2 | 70%甲醇水溶液 methanol solution | 8 | 30 | 常温 room temp. | 本研究 this study |
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