银杏种仁分离蛋白水解产物的制备及其抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2024.02.004

Abstract

Abstract:

Ginkgo biloba seed protein isolate(GBSPI) was hydrolyzed by alcalase, bromelain, and Aspergillus oryzae protease, and the antioxidant activity of the hydrolysates was evaluated through in vitro DPPH radical, hydroxyl radical, and superoxide anion radical scavenging experiments. The results showed that the yields of hydrolysates by alcalase, bromelain, and Aspergillus oryzae protease were 93.60%, 60.00% and 46.80%, with degrees of hydrolysis were 13.76%, 6.28% and 5.55%, respectively. The hydrolysates from three enzymes exhibited good inhibitory effects on DPPH radical, hydroxyl radical, and superoxide anion radical. Among them, the hydrolysate from alcalase showed the strongest antioxidant activity, with IC₅₀ values of 5.29 g/L for DPPH radical, 0.35 g/L for hydroxyl radical, and 7.01 g/L for superoxide anion radical, respectively.

Key words: Ginkgo biloba seed, hydrolysate, hydrolysis degree, antioxidant activity

CLC Number:

TQ35

Xiang WANG, Caihong ZHANG, Jianxin JIANG, Pujun XIE, Lixin HUANG. Preparation of Hydrolysates from Ginkgo biloba Seed Protein Isolate and Their Antioxidant Activity[J]. Chemistry and Industry of Forest Products, 2024, 44(2): 27-32.

Figures/Tables 2

Table 1

Fig.1

References 28

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氨基酸 amino acid		GBSPI	不同酶水解产物different enzymatic hydrolysates
氨基酸 amino acid		GBSPI	碱性蛋白酶 alcalase	菠萝蛋白酶 bromelain	米曲霉蛋白酶 Aspergillus oryzae protease
必需氨基酸 essential amino acids	苏氨酸threonine(Thr)	4.01±0.11	4.96±0.05	4.30±0.11	3.57±0.08
	缬氨酸valine(Val)^*	4.67±0.14	4.58±0.17	3.99±0.15	3.65±0.04
	蛋氨酸methionine(Met)^*	1.40±0.06	1.43±0.13	0.70±0.03	1.49±0.01
	异亮氨酸isoleucine(Ile)^*	3.69±0.08	3.88±0.16	3.43±0.04	3.06±0.09
	亮氨酸leucine(Leu)^*	6.35±0.04	7.18±0.02	6.21±0.12	5.12±0.12
	苯丙氨酸phenylalanine(Phe)^*	3.21±0.05	3.59±0.01	3.21±0.11	2.74±0.15
	赖氨酸lysine(Lys)	3.18±0.10	3.65±0.08	3.51±0.18	2.93±0.09
	组氨酸histidine(His)	2.02±0.12	2.37±0.06	1.82±0.02	1.93±0.05
	色氨酸tryptophan(Trp)	0.76±0.01	0.68±0.07	0.14±0.02	0.38±0.02
非必需氨基酸 non-essential amino acids	天冬氨酸aspartic acid(Asp)	8.09±0.20	10.37±0.14	9.03±0.25	7.40±0.20
	丝氨酸serine(Ser)	3.43±0.22	5.22±0.20	4.49±0.16	3.65±0.04
	谷氨酸glutamic acid(Glu)	8.79±0.24	15.84±0.20	13.28±0.42	9.91±0.20
	甘氨酸glycine(Gly)^*	3.57±0.11	4.17±0.12	3.57±0.02	2.89±0.11
	胱氨酸cystine(Cys)	0.08±0.01	0.30±0.01	0.37±0.01	0.20±0.01
	酪氨酸tyrosine(Tyr)	3.18±0.14	3.34±0.08	3.13±0.13	2.73±0.05
	脯氨酸proline(Pro)^*	2.37±0.06	2.80±0.15	2.84±0.02	2.91±0.13
	精氨酸arginine(Arg)	1.08±0.10	7.80±0.22	10.11±0.11	10.78±0.17
	丙氨酸alanine(Ala)^*	4.79±0.11	3.82±0.18	4.53±0.04	4.73±0.08
疏水氨基酸hydrophobic amino acids		30.05±0.64	31.45±0.90	28.48±0.58	26.59±0.74

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Preparation of Hydrolysates from Ginkgo biloba Seed Protein Isolate and Their Antioxidant Activity

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