纤维素酶辅助水解油茶饼粕提取糖萜素及其产品验证和活性测定

doi:10.3969/j.issn.0253-2417.2024.02.009

Abstract

Abstract:

The extraction of saccharicterpenin from Camellia oleifera cake meal by cellulase hydrolysis method was studied, with the saccharicterpenin yield as the investigation target. An orthogonal experiment was conducted to explore the effects of cellulase dosage, extraction time, liquid-to-solid ratio, extraction temperature, and pH value on the saccharicterpenin yield. The results showed that under the optimal experimental conditions of a solid-to-liquid of 1 ∶ 15(g ∶ mL), cellulase dosage of 4%, enzymatic hydrolysis pH value of 5.0, extraction temperature of 50 ℃, and extraction time of 120 min, the saccharicterpenin yield was 18.39%. The extract was found to conform to the basic structural characteristics of saccharicterpenin through infrared spectroscopy and high-performance liquid chromatography. Identification and component determination of saccharicterpenin were performed according to the national standard method of GB/T 25247—2010. The mass fractions of total sugars and total saccharicterpenin were more than 30%, and the mass fraction of proteins exceeded 7%. Saccharicterpenin exhibited good DPPH radical scavenging ability, with a clearance rate of 90.28% at a mass concentration of 1 g/L. And there was no hemolytic activity at mass concentration less than 5 mg/L, which had good prospects for application as a feed additive.

Key words: Camellia oleifera cake, cellulase, saccharicterpenin, antioxidant activity

CLC Number:

TQ35

Leping ZHANG, Yuejie QIU, Chunrui HAN, Jianxin JIANG. Cellulase-assisted Hydrolysis of Camellia oleifera Seed Meal to Extract Saccharicterpenin and Its Product Validation[J]. Chemistry and Industry of Forest Products, 2024, 44(2): 64-70.

Figures/Tables 5

Table 1

Table 2

Fig.1

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References 21

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实验号No.	A 酶用量/%cellulase dosage	B pH值pH value	C 提取温度/℃extraction temperature	D 提取时间/minextraction time	得率/%yield
1	2	4.5	50	80	15.22
2	2	5.0	55	100	15.87
3	2	5.5	60	120	16.30
4	3	5.5	50	100	18.02
5	3	4.5	55	120	17.49
6	3	5.0	60	80	17.86
7	4	5.0	50	120	18.41
8	4	5.5	55	80	17.77
9	4	4.5	60	100	17.36
k₁	15.80	16.69	17.22	16.95
k₂	17.79	17.38	17.04	17.08
k₃	17.85	17.36	17.17	17.40
R	2.05	0.69	0.17	0.45

实验号No.	总糖/%total sugar	总皂苷/%saponins	蛋白质/%protein
1	42.39±0.26	36.98±0.22	7.42±0.07
2	37.17±0.36	36.90±0.15	8.30±0.31
3	37.96±0.27	40.66±0.28	8.08±0.25
4	40.92±0.14	40.35±0.31	7.63±0.10
5	32.89±0.29	35.39±0.33	8.28±0.13
6	32.24±0.08	42.02±0.13	8.24±0.03
7	37.19±0.10	39.91±0.11	8.69±0.25
8	35.72±0.23	37.19±0.10	8.47±0.02
9	46.04±0.22	38.58±0.34	7.16±0.10

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Cellulase-assisted Hydrolysis of Camellia oleifera Seed Meal to Extract Saccharicterpenin and Its Product Validation

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