Abstract: To investigate the production of lignocellulose-degrading enzyme system from Pleurotus sp. GH196, isolated from a rotten bamboo, submerged fermentation of this fungus was performed under shaking and static conditions. The result showed that lignolytic enzymes produced by GH196 are composed of laccase and manganese peroxidase. Shaking could promote the mycelium growth of the fungus and synthesis of cellulase and xylanase, among them xylanase is the master polysaccharide hydrolase. The xylanase activity can reach 44.7 U/mL when GH196 was cultivated by using rice straw and wheat bran as compound carbon source under 150 r/min shaking condition.

Key words: Pleurotus sp., xylanase, cellulase, lignolytic enzyme