白骨壤中性糖蛋白的分离鉴定及生物活性研究

›› 2008, Vol. 28 ›› Issue (2): 27-32.

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Studies on Isolation, Identification and Biological Activitiy of A Neutral Glycoprotein from the Stems of Avicennia marina(Forsk.)Vierh.

FANG Xu-bo¹, CHEN Xiao-e¹, JIANG Bo², WANG Xiao-lan³, YUAN Yao-ping³

1. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China;2. The Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, China;3. Medical Faculty, Southern Yangtze University, Wuxi 214036, China

Received:2007-04-26 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30

Abstract

Abstract: HAM-3-IIa, a neutral polysaccharide fraction, was obtained from Avicennia marina(Forsk.) Vierh. by a series steps of extraction, isolation and purification. The purified HAM-3-IIa was proved to be homogenous by HPGFC and gel column chromatography. Its molecular weight was 97600. HAM-3-IIa was regarded as neutral glycoprotein with 4.9% protein and 95% carbohydrate. The carbohydrate fraction was composed of arabinose, galactose and xylose. Linkage between sugars and amino acids was shown to be O-glycol-Ser bond by β-elimination reactions. HAM-3-IIa expressed potent anti-complementary activity via classical and alternative pathways with the increase of activity proportional to dosage. It was confirmed from the study that the activity of polysaccharide was not due to the presence of microbial contamination during the purification processes.

Key words: Avicennia marina(Forsk.) Vierh., polysaccharide, anti-complementary activity

CLC Number:

TQ91

FANG Xu-bo;CHEN Xiao-e;JIANG Bo;WANG Xiao-lan;YUAN Yao-ping. Studies on Isolation, Identification and Biological Activitiy of A Neutral Glycoprotein from the Stems of Avicennia marina(Forsk.)Vierh.[J]. , 2008, 28(2): 27-32.

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Studies on Isolation, Identification and Biological Activitiy of A Neutral Glycoprotein from the Stems of Avicennia marina(Forsk.)Vierh.

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