Abstract: Immobilized amylases were prepared by using polymer of maleated rosin acid-hexandiol ester (PMHE), copolymer of maleated rosin acid-glycol-acrylic acid ester-acrylic acid and polymer of maleated rosin acid-glycol-acrylic acid ester (PMGAE) respectively as carriers and the method of metallic ion coordination. The performance of the immobilized enzyme was determined. The mechanism of immobilization was discussed. The result showed that PMGAECa(Ⅱ) and poly(MGAE-AA)Ca(Ⅱ) were the best carriers for immobilizing amylase among all carriers with metal ions as coordination bridge. Their activities were maintained at 11.33 and 9.44mg/g for per 5min and relative retention activities were 32.78% and 37.59%, respectively, after using five times. The optimal reaction conditions of starch oxidation catalyzed by the amylase immobilized on PMGAECa(Ⅱ) and poly(MGAE-AA)Ca(Ⅱ)were found to be as follows: reaction temperature 60℃(20℃ higher than that of free enzyme) and 50℃(10℃ higher than that of free enzyme), pH value 6.03 and 5.00, respectively.

Key words: rosin-based functional polymer, immobilized enzyme, amylase, activity