漂洗降低白及militarine含量的响应面法研究

doi:10.3969/j.issn.0253-2417.2022.06.016

Abstract

Abstract:

In order to establish a simple and effective processing method to reduce the bitterness of Bletilla striata (Thunb. ex A. Murray) Rchb.f., response surface methodology was used to explore the best optimum conditions of hot water rinsing B. striata fresh stem slices. Taking the content of 1, 4-bis[4-(glucoseoxy) benzyl]-2-isobutyl malate(militarine) as the index the effects of hot water rinsing temperature, rinsing time and pH value on the militarine content in B. striata were investigated by a single factor experiment, and the rinsing was optimized by Box-Behnken response surface analysis. The optimum conditions were obtained as follows: rinsing temperature of 95 ℃, rinsing time of 50 min, hot water pH of 6.5.Under these optimized conditions, the mass fraction of militarine decreased by 63.55% in parallel experiments, and the mass fraction of militarine in the fresh stem slices of B. striata reduced 83.10% after three rinses. Rinsing B. striata fresh stem slices with hot water for 1-3 times could significantly reduce the content of militarine under optimum conditions.

Key words: Bletilla striata, militarine, hot water rinsing, response surface methodology

CLC Number:

TQ35
R284.1

Moran CHEN, Huazhong YU, Leilei LIU, Kehua TANG. Application of Response Surface Methodology for Optimication of Reducing the Content of Militarine in Bletilla striata by Rinsing[J]. Chemistry and Industry of Forest Products, 2022, 42(6): 117-122.

Figures/Tables 3

Fig.1

Table 1

Table 2

References 17

1	梁宇轩, 何晓梅, 朱富成, 等. 白及主要生物活性物质及药理作用研究进展[J]. 湖南农业科学, 2018, (3): 107- 109.
	LIANG Y X , HE X M , ZHU F C , et al. The main bioactive substance and pharmacology of Bletilla striata[J]. Hunan Agricultural Sciences, 2018, (3): 107- 109.
2	孙爱静, 庞素秋, 王国权. 中药白及化学成分与药理活性研究进展[J]. 环球中医药, 2016, 9 (4): 507- 511. doi: 10.3969/j.issn.1674-1749.2016.04.038
	SUN A J , PANG S Q , WANG G Q . Advances of chemical constituents and pharmacological activities of Bletilla striat[J]. Traditional Chinese Medicine, 2016, 9 (4): 507- 511. doi: 10.3969/j.issn.1674-1749.2016.04.038
3	JIANG S , WANG M Y , YUAN H W , et al. Medicinal plant of Bletilla striata: A review of its chemical constituents, pharmacological activities, and quality control[J]. World Journal of Traditional Chinese Medicine, 2020, 6 (4): 393- 407. doi: 10.4103/wjtcm.wjtcm_58_20
4	陈黎, 黄良永, 毛闪闪, 等. 鄂西北地区白及高效液相指纹图谱研究[J]. 中国医院药学杂志, 2014, 34 (2): 105- 109.
	CHEN L , HUANG L Y , MAO S S , et al. Study on the HPLC fingerprint of Bletilla striata from northwest area of Hubei provine[J]. Chinese Journal of Hospital Pharmacy, 2014, 34 (2): 105- 109.
5	JI X L, YIN M S, NIE H, et al. A review of isolation, chemical properties, and bioactivities of polysaccharides from Bletilla striata[J/OL]. BioMed Research International, 2020, 2020: 5391379[2021-07-26]. https://doi.org/10.1155/2020/5391379.
6	颜智, 刘刚, 刘育辰, 等. 白及化学成分、药理活性及质量评价研究进展[J]. 广州化工, 2018, 46 (16): 42- 44. 42-44, 48 doi: 10.3969/j.issn.1001-9677.2018.16.019
	YAN Z , LIU G , LIU Y C , et al. Research progress on chemical constitunets, pharmacological activities and quality evaluation of Bletilla striata[J]. Guangzhou Chemical Industry, 2018, 46 (16): 42- 44. 42-44, 48 doi: 10.3969/j.issn.1001-9677.2018.16.019
7	陈曦. 几种云南道地中药类保健食品市场现状分析与营销定位[D]. 重庆: 西南大学, 2012.
	CHEN X. The current situation analysis and marketing positioning of genuine medicinal material health foods in Yunnan area[D]. Chongqing: Southwest University, 2012.
8	李美英, 姜雨, 余超. 2013~2015年中药类保健食品批准现况及思考[J]. 中南药学, 2016, 14 (10): 1139- 1142. doi: 10.7539/j.issn.1672-2981.2016.10.028
	LI M Y , JIANG Y , YU C . Perspective on the approved health food containing Chinese herbal medicine from 2013 to 2015[J]. Central South Pharmacy, 2016, 14 (10): 1139- 1142. doi: 10.7539/j.issn.1672-2981.2016.10.028
9	吴梅, 田守征, 郑永仁, 等. 白及苦味成分的分离、鉴定及抗炎药效学初研[J]. 时珍国医国药, 2019, 30 (2): 372- 374.
	WU M , TIAN S Z , ZHENG Y R , et al. Isolation, identification and preliminary study on anti-infla mmatory pharmacodynamics of Bletilla striata bitter flavor components[J]. Lishizhen Medicine and Materia Medica Research, 2019, 30 (2): 372- 374.
10	代丽凤, 罗理勇, 罗江琼, 等. 植物苦味物质概况及其在食品工业的应用[J]. 中国食品学报, 2020, 20 (11): 305- 318.
	DAI L F , LUO L Y , LUO J Q , et al. Overview of bitter substances in plants and their application in food industry[J]. Journal of Chinese Institute of Food Science and Technology, 2020, 20 (11): 305- 318.
11	李敏, 郭顺星, 王春兰, 等. 2-异丁基苹果酸葡萄糖氧基苄酯类化合物在兰科植物中的分布特点及药理活性[J]. 中国药学杂志, 2010, 45 (10): 724- 726.
	LI M , GUO S X , WANG C L , et al. Distribution characteristics and pharmacological activities of glucosyloxybenzyl 2-isobutylmalates in Orchids[J]. Chinese Pharmaceutical Journal, 2010, 45 (10): 724- 726.
12	国家药典委员会. 中华人民共和国药典: 一部[M]. 2020版北京: 中国医药科技出版社, 2020.
	Chinese Pharmacopoeia Commission . Pharmacopoeia of the People's Republic of China: Part 1[M]. 2020 ed Beijing: China Medical Science Press, 2020.
13	王贵民, 董振红, 郝再彬. 甜叶菊糖苷的应用和安全性的研究进展[J]. 中国食品添加剂, 2007, (6): 65- 69.
	WANG G M , DONG Z H , HAO Z B . Application and safety of steviolglycoside in leaves of Stevia rebaudiana(bertoni) bertoni[J]. China Food Additives, 2007, (6): 65- 69.
14	刘娜, 袁金凤, 彭诗涛, 等. 基于militarine含量结合UPLC指纹图谱的不同产地白及饮片质量分析[J]. 天津中医药, 2020, 37 (5): 583- 589.
	LIU N , YUAN J F , PENG S T , et al. Quality analysis of Bletilae Rhizoma slices from different producing areas based on the militarine content determination and establishment of fingerprint by UPLC[J]. Tianjin Journal of Traditional Chinese Medicine, 2020, 37 (5): 583- 589.
15	唐修静, 张羽斌, 文运, 等. 不同炮制方法对白及中militarine及浸出物含量的影响[J]. 中国实验方剂学杂志, 2014, 20 (7): 67- 69.
	TANG X J , ZHANG Y B , WEN Y , et al. Different effect with different processing methods on chemical constituents of Bletillae Rhizoma[J]. China Journal of Experimental Traditional Medical Formulae, 2014, 20 (7): 67- 69.
16	段营辉, 黄澜, 朱樵苏, 等. 基于液质联用法对白及中2-异丁基苹果酸葡萄糖氧基苄酯类成分的定性定量分析[J]. 中国医院药学杂志, 2020, 40 (9): 1009- 1013.
	DUAN Y H , HUANG L , ZHU Q S , et al. Qualitative and quantitative analysis of 2-isopropyl-(glucosyloxy) benzyl malate in Bletilla striata by UPLC-ESI-Q/TOF-MF[J]. Chinese Journal of Hospital Pharmacy, 2020, 40 (9): 1009- 1013.
17	周美, 万科, 马风伟, 等. 响应面法优化白及多糖酶解工艺及其抗氧化、免疫活性研究[J]. 食品研究与开发, 2020, 41 (10): 128- 135.
	ZHOU M , WAN K , MA F W , et al. Response surface methodology for optimization of enzymatic hydrolysis on Bletilla striata(Thunb.) Reiehb.f. polysaccharides and antioxidant, in vitro immunostimulatory activities analysis[J]. Food Research and Development, 2020, 41 (10): 128- 135.

试验号 No.	X₁ 漂洗温度/℃ rinsing temp.	X₂ 漂洗时间 rinsing time	X₃ 漂洗液pH值 rinsing solution pH value	militarine下降比例/% militarine decrease ratio
1	90	30	6.5	47.58
2	90	40	8.0	64.97
3	90	50	6.5	63.19
4	90	40	8.0	61.09
5	80	40	9.5	41.38
6	90	40	8.0	55.34
7	90	40	8.0	57.04
8	90	40	8.0	58.96
9	80	30	8.0	40.83
10	100	40	6.5	59.05
11	100	30	8.0	55.16
12	100	40	9.5	54.78
13	80	50	8.0	47.56
14	100	50	8.0	62.42
15	90	50	9.5	53.06
16	90	30	9.5	53.56
17	80	40	6.5	49.85

方差来源 variance source	平方和 sum of squares	自由度 degree of freedom	均方差 mean square	F值 F value	P值 P value	显著性 significance
模型model	748.26	9	83.14	8.21	0.005 6	**
X₁	335.28	1	335.28	33.10	0.000 7	**
X₂	105.85	1	105.85	10.45	0.014 4	*
X₃	35.66	1	35.66	3.52	0.102 7
X₁X₂	0.07	1	0.070	6.933×10^-3	0.936 0
X₁X₃	4.41	1	4.41	0.44	0.530 5
X₂X₃	64.88	1	64.88	6.41	0.039 2	*
X₁²	128.99	1	128.99	12.73	0.009 1	**
X₂²	25.33	1	25.33	2.50	0.157 8
X₃²	30.24	1	30.24	2.99	0.127 6
残差residual	70.90	7	10.13
失拟误差lack of fit	14.81	3	4.94	0.35	0. 791 3
纯误差pure error	56.10	4	14.02
总离差total dispersion	819.16	16

[1]	Tian HUANG, Xiaotong ZHANG, Jianglin ZHAO, Jianming GUO, Xin ZHOU, Yong XU. Preparation of Xylooligosaccharides by Xylonic Acid Assisted Hydrolysis of Xylan and Its Separation and Recovery Process [J]. Chemistry and Industry of Forest Products, 2021, 41(5): 8-14.
[2]	Shuqin CHEN, Yanyan YE, Yingxuan QUAN, Linhai LI, Lin NI, Shuangquan ZOU. Extraction Process and Components Analysis of Oil from the Seeds of Euscaphis konishii Hayata [J]. Chemistry and Industry of Forest Products, 2021, 41(1): 53-59.
[3]	Yiwei JIANG, Na GUO, Zhiguo LIU, Chunjian ZHAO, Yujie FU, Xiqing WANG. Optimization of Extraction Technology of Blue Honeysuckle Seed Oil and Analysis of Its Fatty Acid Composition [J]. Chemistry and Industry of Forest Products, 2020, 40(6): 30-36.
[4]	Aihua ZHANG,Juan TANG,Pengying LAI,Yidan HE,Jilie LI,Zhihong XIAO. Bio-hydrocarbon Fuel Preparation from Pyrolysate of Swida wilsoniana Fruit Oil by Catalytic Hydrogenation [J]. Chemistry and Industry of Forest Products, 2020, 40(5): 43-49.
[5]	Tingting ZHANG,Xiuli HAN,Ying LIU,Chunling DONG,Chun CHANG. Adsorption Characteristics of Ciprofloxacin by Biomass-based Activated Carbon [J]. Chemistry and Industry of Forest Products, 2020, 40(4): 71-78.
[6]	Liang CHEN,Guiping ZHAN,Ying LU,Jiaxing LI,Ji YU,Chengjin MA,Maojun YAO. Preparation and Stability of Eucommia ulmoides Oliv.Seed Oil Liposomes [J]. Chemistry and Industry of Forest Products, 2020, 40(1): 68-76.
[7]	Zhexuan LÜ,Weiwei ZHANG,Dandan LIU,Liwei ZHU,Jianxin JIANG. Extraction Technology and Surface Tension Characterization of Gymnocladus chinensis Saponin [J]. Chemistry and Industry of Forest Products, 2019, 39(6): 102-108.
[8]	Yan PENG,Kehua TANG,Aiwen DONG. Purification of Bletilla striata Polyphenols by Macroporous Resin and Its Antioxidant Activities in Vitro [J]. Chemistry and Industry of Forest Products, 2019, 39(4): 91-99.
[9]	LIU Dandan, HAN Weifan, JIANG Jianxin, ZHU Liwei. Technology of Microwave-assisted Extraction of Sapindus Peel Polysaccharide and Its Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2018, 38(4): 109-116.
[10]	LIU Sha, CAI Yi-xi, FAN Yong-sheng, LI Xiao-hua, WANG Jia-jun. Optimization of Online Upgrading of Bio-oil with MCM-41 and Catalyst Durability Analysis [J]. Chemistry and Industry of Forest Products, 2016, 36(4): 55-63.
[11]	XU Xiao-xiang, YIN Xiao-li, HONG Yan-ping, ZOU Kai, SHANGGUAN Xin-chen, LIANG Qiang. Extraction Process and Antioxidant Activity of Pinus taeda L. Polysaccharides [J]. Chemistry and Industry of Forest Products, 2016, 36(3): 114-120.
[12]	LIU Tong-fang, YU Hua-zhong, CHEN Yan-mei, WANG Ting. Optimization of Semisynthetic Myricetin from Dihydromyricetion by Response Surface Process [J]. Chemistry and Industry of Forest Products, 2015, 35(4): 125-130.
[13]	TIAN Ning, XIAN Mo, HU Yang-dong, LIU Wei. Optimization of Medium for Production of Geraniol by the Recombinant Escherichia coli [J]. Chemistry and Industry of Forest Products, 2015, 35(4): 131-137.
[14]	LI He, LIN Ri-hui, HUANG Wen-qin, LONG Han, LIU Zuo-jiao, SU Jia-ting. Catalytic Synthesis of Rosin Starch Ester by Immobilized Lipase [J]. Chemistry and Industry of Forest Products, 2015, 35(2): 103-110.
[15]	FAN Yong-sheng, CAI Yi-xi, LI Xiao-hua, YU Ning, ZHANG Rong-xian, YIN Hai-yun. Vacuum Pyrolysis of Camphorwood Sawdust Optimized by Response Surface Methodology and Bio-oil Composition Analysis [J]. Chemistry and Industry of Forest Products, 2014, 34(6): 29-36.

Application of Response Surface Methodology for Optimication of Reducing the Content of Militarine in Bletilla striata by Rinsing

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 3

References 17

Related Articles 15

Recommended Articles

Metrics

Comments