簕竹属竹叶中黄酮类成分分析及其抗氧化活性

doi:10.3969/j.issn.0253-2417.2023.01.012

Abstract

Abstract:

To explore the development and utilization of flavonoids in the leaves of the main bamboo species of Bambusa, extracts of leaves from ten bamboo species were obtained by extraction, separation and purification, using bamboo leaves from Bambusa as the materials. A HPLC method was established for the determination of 5 flavonoids in extract of bamboo leaves, and the antioxidant activities of those extracts were evaluated by DPPH and ABTS assays. The HPLC method for the determination of 5 flavonoids isoorientin, orientin, vitexin, isovitexin and luteoside in extract of bamboo leaves was using YMC-Pack ODS-A column(250 mm×4.6 mm, 5 μm) with 0.5% acetic acid/water(A) and acetonitrile(B) as the mobile phases, elution with 14% B for 40 min, the injection volume 10 μL, the flow rate 1 mL/min, the column temperature 30 ℃ and the detection wavelength at 340 nm. Under these conditions, the five flavonoids were completely separated within 40 min, the degree of separation was greater than 1.2, and the concentration had a good linear correlation with the peak area(R²>0.999). The recovery rate was 94.36%-105.37%, and the relative standard deviation(RSD) was 0.98%-3.48%. These results indicated that the method was stable and reliable. The total contents of 5 flavonoids in extract of leaves from 10 bamboo species of Bambusa measured under these conditions were 62.30-223.24 mg/g, and the mass fraction of bamboo leaves extract from B. multiplex f. fernleaf was the highest(223.24 mg/g). The half inhibitory mass fraction(IC₅₀) values of DPPH· and ·ABTS⁺ were 28.58-44.37 mg/L and 8.58-11.07 mg/L, respectively, slightly higher than those of Vc and BHT. The bamboo species with better DPPH· scavenging rate were B. eutuldoides (IC₅₀=28.58 mg/L), B. textilis (IC₅₀=28.68 mg/L) and B. multiplex f. fernleaf (IC₅₀=31.65 mg/L). The bamboo species with better ABTS^·+ scavenging rate were B. multiplex f. fernleaf(IC₅₀=8.58 mg/L), B. multiplex f. silverstripe(IC₅₀=9.11 mg/L) and B. textilis (IC₅₀=9.27 mg/L). The IC₅₀ values of B. multiplex f. fernleaf and B. textilis of two kinds of radicals were lower, which were the two bamboo species with the best removal effect and the most value of development and utilization.

Key words: HPLC, Bambusa, bamboo leaves extract, flavonoids, antioxidant activity

CLC Number:

TQ35

Rongmiao AN, Ting YUAN, Xuefeng GUO. Quantification and Antioxidant Activity of Flavonoids in Bamboo Leaves Extract of Bambusa[J]. Chemistry and Industry of Forest Products, 2023, 43(1): 97-103.

Figures/Tables 8

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References 16

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编号 No.	竹子种类 species of bamboo
1	凤尾竹B. multiplex f. fernleaf (R. A. Young) T. P. Yi
2	小琴丝竹B. multiplex (Lour.) Raeusch. ex Schult. et Schult. f. ‘Alphonse-Karr’
3	青皮竹B. textilis McClure
4	桃枝竹B. glaucescens (Willd.) Siebold ex Munro var. shimadai (Hayata) L. C. Chia et But
5	河边竹B. multiplex T. H. Wen
6	黄金间碧竹B. vulgaris Schrad.ex J. C. Wendl. ‘Vittata’
7	小佛肚竹B. ventricosa McClure ‘Nana’
8	大眼竹B. eutuldoides McClure
9	凤凰竹B. multiplex (Lour.) Raeusch. ex Schult. et Schult. f.
10	银丝竹B. multiplex f. silverstripe (R. A. Young) T. P. Yi

标准品 standard	线性方程 linear equation	线性范围/(mg·L^-1) linear range	R²	LOD/(mg·L^-1)	LOQ/(mg·L^-1)
异荭草苷isoorientin	y=16 434x-5 267.7	6.0~120.0	0.999 7	0.25	1.60
荭草苷orientin	y=15 808x-9 139.2	5.5~110.0	0.999 8	0.50	1.33
牡荆苷vitexin	y=22 502x-13 409.0	5.5~110.0	0.999 5	0.50	1.82
异牡荆苷isovitexin	y=24 240x-10 801.0	5.5~110.0	0.999 4	0.50	1.20
木犀草苷luteoloside	y=15 291x-42 563.0	5.0~100.0	0.999 8	1.00	3.00

化合物 compound	精密度/% precision	日内稳定性/% intra-day	日间稳定性/% inter-day
异荭草苷isoorientin	0.09	1.57	2.60
荭草苷orientin	0.39	1.73	3.15
牡荆苷vitexin	0.46	3.17	2.08
异牡荆苷isovitexin	0.22	1.23	1.16
木犀草苷luteoloside	0.92	2.72	3.62

成分 compound	样品质量/mg sample mass	成分含有量/μg content	标准品加入量/μg addition	测得量/μg measured	回收率/% recovery	平均回收率/% average recovery	RSD/%
异荭草苷 isorientin	2.12	78.94		128.88	95.71
	2.13	79.31	55.71	127.15	94.17	94.36	1.27
	2.28	84.90		131.04	93.20
荭草苷 orientin	2.35	4.87		8.82	101.84
	1.97	4.09	3.79	7.85	99.66	98.84	3.48
	2.27	4.71		8.07	95.02
牡荆苷 vitexin	2.35	3.99		8.57	107.49
	1.97	3.34	3.98	7.67	104.64	105.37	3.26
	2.27	3.85		7.91	100.98
异牡荆苷 isovitexin	2.12	122.23		222.97	100.61
	2.13	122.80	99.38	219.26	98.68	99.54	0.98
	2.28	131.45		229.29	99.33
木犀草苷 luteoloside	2.12	17.54		40.71	103.45
	2.13	14.71	21.80	36.14	98.98	99.97	3.11
	2.28	16.95		37.78	97.48

竹种 Bamboo species	异荭草苷 isorientin	荭草苷 orientin	牡荆苷 vitexin	异牡荆苷 isovitexin	木犀草苷 luteoloside	总量 total
凤尾竹B. multiplex	122.99±2.00^a	1.08±0.05^c	0.90±0.10^e	84.65±1.59^a	13.62±0.45^a	223.24±3.87^a
小琴丝竹B. multiplex	29.19±0.27^h	0.87±0.02^d	1.08±0.11^d	28.34±0.26^g	2.82±0.15^g	62.30±0.40^g
青皮竹B. textilis	50.12±0.28^f	0.77±0.06^de	—	50.42±0.24^d	5.61±0.17^d	106.92±0.43^c
桃枝竹B. glaucescens	24.74±0.67ⁱ	0.62±0.15^e	0.67±0.05^e	40.20±0.92^e	5.00±1.77^e	71.23±1.57^f
河边竹B. multiplex	61.64±0.24^d	1.75±0.45^b	1.64±0.07^bc	32.36±0.26^f	3.49±0.05^f	100.88±0.14^d
黄金间碧竹B. vulgaris	19.03±0.34^j	0.61±0.50^e	1.81±0.06^b	40.65±0.24^e	4.64±0.42^e	66.74±0.91^fg
小佛肚竹B. ventricosa	37.24±2.69^g	2.07±0.06^a	1.70±0.17^bc	57.65±0.91^c	7.47±0.04^c	106.13±2.05^c
大眼竹B. eutuldoides	66.85±3.68^c	1.14±0.29^c	2.64±0.06^a	21.83±0.75^g	2.60±0.12^g	95.06±4.03^e
凤凰竹B. multiplex	86.82±2.62^b	1.11±0.20^c	1.01±0.09^de	60.36±2.25^b	8.93±0.23^b	158.23±4.96^b
银丝竹B. multiplex	57.00±1.99^e	1.70±0.14^b	1.53±0.14^c	35.56±1.28^f	3.62±0.19^f	99.41±3.57^de

Quantification and Antioxidant Activity of Flavonoids in Bamboo Leaves Extract of Bambusa

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样品 sample	IC₅₀/(mg·L^-1)
样品 sample	DPPH·	·ABTS⁺
Vc	5.77	2.29
BHT	8.30	2.31
凤尾竹B. multiplex	31.65	8.58
小琴丝竹B. multiplex	39.02	10.57
青皮竹B. textilis	28.68	9.27
桃枝竹B. glaucescens	44.37	10.51
河边竹B. multiplex	34.38	10.53
黄金间碧竹B. vulgaris	38.73	9.32
小佛肚竹B. ventricosa	38.59	11.07
大眼竹B. eutuldoides	28.58	9.70
凤凰竹B. multiplex	34.48	10.29
银丝竹B. multiplex	35.82	9.11

自由基 free radical	异荭草苷 isorientin	荭草苷 orientin	牡荆苷 vitexin	异牡荆苷 isovitexin	木犀草苷 luteoloside	总量 total
DPPH·	-0.620^*	-0.093	-0.072	-0.132	-0.158	-0.468
·ABTS⁺	-0.476	-0.310	-0.121	-0.314	-0.319	-0.448

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