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Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (1): 34-42.doi: 10.3969/j.issn.0253-2417.2023.01.004

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Highly Effective Separation of Paclitaxel by Using Core-shell Rosin-based Polymer Bonded Silica as Liquid Chromatography Stationary Phase

Hao LI, Wenbo XIE, Lei ZENG, Liuyu YI, Wen LI, Fuhou LEI()   

  1. School of Chemistry and Chemical Engineering, Guangxi Minzu University; Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission; Guangxi Key Laboratory of Chemistry and Engineering of Forest Products; Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Nanning 530006, China
  • Received:2021-11-17 Online:2023-02-28 Published:2023-02-28
  • Contact: Fuhou LEI E-mail:leifuhougxun@126.com


A rosin-based core-shell polymer bonded silica(RP@SiO2) stationary phase was prepared by the radical polymerization of fumaropimaric acid tri(β-acryloxyl ethyl) ester(FATE) onto alkylated silica(TPM-SiO2). The RP@SiO2 stationary phase was characterized and evaluated, the separation performance and mechanism of RP@SiO2 column for paclitaxel(PTX) and its analogs were discussed, and it was used for the separation and purification of PTX from crude yew-bark extract. The results showed that FATE was successfully bonded to the surface of silica, the core-shell structure material was successfully prepared, and the average particle size of RP@SiO2 was about 5 μm. The RP@SiO2 column was a typical reversed-phase column similar to C18 column, showing excellent chromatographic performance, satisfactory performance reproducibility, and typical reversed-phase chromatographic behavior. The RP@SiO2 column was used to separate paclitaxel analogs, and the separation degree of continuous elution samples was more than 6.6. Stoichiometric displacement theory for retention(SDT-R) and van't Hoff thermodynamic analysis showed that hydrophobic interactions determined the analyte retention, and the separation of paclitaxel analogs on RP@SiO2 column was an exothermic process driven by enthalpy. Furthermore, SDT-R could be used to explain its retention mechanism. The RP@SiO2 column was employed to separate and purify paclitaxel from crude yew-bark extract, increasing paclitaxel purity from 6% to 81%.

Key words: rosin-based stationary phase, HPLC, retention mechanism, paclitaxel

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