HPLC法测定乌韭的荭草苷和牡荆素含量

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (5): 103-106.

HPLC法测定乌韭的荭草苷和牡荆素含量

任冰如, 夏冰, 李维林, 吴菊兰, 张涵庆

江苏省中国科学院植物研究所;江苏省迁地保护重点实验室, 江苏南京 210014

收稿日期:2006-11-23 修回日期:1900-01-01 出版日期:2007-10-30 发布日期:2007-10-30

Determination of Orientin and Vitexin in Stenoloma chusanum by HPLC

REN Bing-ru, XIA Bing, LI Wei-lin, WU Ju-lan, ZHANG Han-qing

The Provincial Key Laboratory for Plant Ex Situ Conservation;Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

Received:2006-11-23 Revised:1900-01-01 Online:2007-10-30 Published:2007-10-30

摘要/Abstract

摘要： 建立乌韭地上部分荭草苷和牡荆素含量的测定方法,检测不同产地乌韭中两种黄酮苷的含量,对乌韭资源进行评价.用超声波提取法提取,反相高效液相色谱法(RP-HPLC)测定,GeminiC₁₈色谱柱,乙腈/1%磷酸溶液(体积比15:85)为流动相,流速1 mL/min,检测波长340nm.荭草苷在0.025～0.18μg范围内有良好的线性关系,加样回收率为100.61%,相对标准偏差(RSD)2.18%;牡荆素在0.035～0.207 μg范围内有良好的线性关系,加样回收率为99.77%,RSD 2.37%.云南丘北所产乌韭的荭草苷和牡荆素质量分数都是最高,分别达到0.1166%和0.157%,移栽到南京地区1年后荭草苷含量下降,牡荆素含量增高.以荭草苷和牡荆素含量为指标,云南丘北所产乌韭是较好的种源.

关键词: 乌韭, 荭萆苷, 牡荆素

Abstract: A method was established for determining contents of orientin and vitexin in Stenoloma chusanum(Linn.) Ching and to measure the contents of the two flavonoids in different plants in order to evaluate this resource by HPLC. Flavonoids were obtained by ultrasonic extraction, separation was carried out on Gemini C₁₈ column with a mixture of acetonitrile/1% phosphoric acid (volume ratio 15:85) as mobile phase at flow rate 1.0 mL/min and detective wavelength 340 nm. Orientin content showed a good linear relationship within 0.025-0.148 μg (R²=0.9995) and the average recovery was100.61%, RSD was 2.18%. Vitexin content showed a good linear relationship also within 0.035-0.207 μg(R²=0.9999) and the average recovery was 99.77%, RSD was 2.37%. The sample of the highest content is from Yunnan Qiubai which contained orientin 0.116 6% and vitexin 0.145 7%. Orientin content decreased but vitexin content increased when the plant was transplanted from Qiubai to Nanjing after one year. Plant from Qiubai is an excellent resource according contents of orientin and vitexin.

Key words: Stenoloma chusanum(Linn. )Ching, orientin, vitexin

中图分类号:

TQ91
Q94

任冰如;夏冰;李维林;吴菊兰;张涵庆. HPLC法测定乌韭的荭草苷和牡荆素含量[J]. 林产化学与工业, 2007, 27(5): 103-106.

REN Bing-ru;XIA Bing;LI Wei-lin;WU Ju-lan;ZHANG Han-qing. Determination of Orientin and Vitexin in Stenoloma chusanum by HPLC[J]. , 2007, 27(5): 103-106.

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