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林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 81-84.

• 研究报告 • 上一篇    下一篇

核桃楸叶乙醇提取物的抑菌活性及活性成分分析

孙墨珑1, 宋湛谦2, 方桂珍3   

  1. 1. 东北林业大学 理学院, 黑龙江 哈尔滨 150040;2. 中国林业科学研究院 林产化学工业研究所;国家林业局 林产化学工程重点开放性实验室, 江苏 南京 210042;3. 东北林业大学 材料科学与工程学院, 黑龙江 哈尔滨 150040
  • 收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20
  • 通讯作者: 宋湛谦,中国工程院院士,研究员,博士生导师,从事生物质资源的化学改性和应用,特别是松脂资源的深加工利用。

Antifungal Activities and Active Components of Alcoholic Extracts from Leaves of Juglans mandshurica Maxim.

SUN Mo-long1, SONG Zhan-qian2, FANG Gui-zhen3   

  1. 1. College of Science, Northeast Forestry University, Harbin 150040, China;2. Institute of Chemical Industry of Forest Products, CAF;Key and Open Lab. on Forest Chemical Engineering, SFA, Nanjing 210042, China;3. College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China
  • Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

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摘要： 采用常规提取法浸提核桃楸叶的抑菌活性成分.研究结果表明,核桃楸叶乙醇提取物及其乙酸乙酯萃取相对杨树叶枯病菌和樟子松枯梢病菌有抑菌活性,核桃楸叶乙醇提取物及其乙酸乙酯萃取相对杨树叶枯病菌的最低抑菌质量浓度(MIC)为3.12 g/L,对樟子松枯梢病菌的MIC为6.25 g/L.采用GC-MS分析了核桃楸叶乙醇提取物中乙酸乙酯萃取相经柱层析后集分Ⅰ和集分Ⅱ的化学组成和相对含量.分析结果表明,乙酸乙酯萃取相中主要活性成分为相对含量较高的5-羟基-1,4-萘醌(胡桃醌)、D-阿洛糖、5,8-二羟基-1,4-萘醌、1,5-萘二酚、2,3-二氢苯并呋喃、7-甲氧基-1-四氢萘酮、1-萘酚、8-羟基-2-甲氧基-1,4-萘醌和4-甲基-2,6-二羟基喹啉等.

关键词: 核桃楸叶, 提取物, 抑菌活性

Abstract: The extracts from leaves of Juglans mandshurica Maxim. were obtained by common extracting method for studying its antifungal activities and analyzing the active components. The results showed that alcoholic extracts and the acetic ether part of alcoholic extracts (separated with acetic ether from alcoholic extracts) from leaves of J. mandshurica had antifungal activities toward Alternaria alternate Fr. and Sphaeropsis sapinea Fr.. MIC of the alcoholic extracts and the acetic ether part of alcoholic extract were both 3.12 g/L toward A. alternate, and both 6.25 g/L toward S. sapinea. The active components in the acetic ether part I and part II (separated with silica gel column chromatography) of alcoholic extracts from leaves of J. mandshurica were analyzed by GC-MS. The results showed that major active components of relatively high content in the acetic ether part of alcoholic extracts from leaves of J. mandshurica were 1,4-naphthalenedione, 5-hydroxy-(juglone), D-allose, 5,8-dihydroxy-1,4-naphthalenedione, 1,5-naphthalenediol, 2,3-dihydro-benzofuran, 7-methoxy-1-tetralone, 1-naphthalenol, 8-hydroxy-2-methoxy-1,4-naphthalenedione and 4-methyl-2,6-dihydroxyquinoline.

Key words: Juglans mandshurica Maxim. leaves, extracts, antifungal activities

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