欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (04): 39-43.

• 论文 • 上一篇    下一篇

应用生物活性追踪法对香椿抗氧化活性的研究

王昌禄1, 江慎华1,2, 陈志强1, 陈勉华1, 王玉荣1, 刘常金1, 周庆礼3, 夏廉法4   

  1. 1. 天津科技大学食品工程与生物技术学院, 天津市食品营养与安全重点实验室, 天津, 300457;2. 江苏大学食品与生物工程学院, 江苏镇江, 212013;3. 天津市食品加工工程中心, 天津, 300457;4. 河南省农业科学研究院, 河南郑州, 450002
  • 收稿日期:2007-06-29 修回日期:1900-01-01 出版日期:2008-08-30 发布日期:2008-08-30

Study on Antioxidative Activity of Toona sinensis(A. Juss.) Roem.Based on Bioassay-guided Method

WANG Chang-lu1, JIANG Shen-hua1,2, CHEN Zhi-qiang1, CHEN Mian-hua1, WANG Yu-rong1, LIU Chang-jin1, ZHOU Qing-li3, XIA Lian-fa4   

  1. 1. Tianjin Key Laboratory of Food Nutrition and Safety;College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China;2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;3. Tianjin Engineering Center of Food Processing, Tianjin 300457, China;4. Henan Academy of Agricultural Science, Zhengzhou 450002, China
  • Received:2007-06-29 Revised:1900-01-01 Online:2008-08-30 Published:2008-08-30

PDF (PC)

637

摘要： 采用生物活性追踪法,将香椿老叶甲醇提取物划分为4个不同极性部位,通过测定其总还原力、1,1-二苯基-2-苦基-肼(DPPH)自由基清除能力和三价铁还原抗氧化能力(FRAP)测试值,确定香椿老叶提取物抗氧化活性及其组成.结果表明:香椿老叶提取物中的强抗氧化活性物质主要集中在乙酸乙酯部位,该部位的总还原力相当于467.3mg/gVc的总还原值,FRAP值相当于10578μmol/g硫酸亚铁的FRAP值,质量浓度50mg/L的DPPH自由基清除率为92.84%,均比同浓度的2,4-二叔丁基甲基苯酚(BHT)强.相关性研究表明,香椿老叶提取物的抗氧化活性主要在于其含有较高的黄酮类化合物所致.TLC和HPLC分析表明:香椿老叶提取物强抗氧化活性物质主要由4个黄酮-糖苷类化合物组成.

关键词: 抗氧化, 香椿, 生物活性追踪法, 评价

Abstract: Methanol extract of old leaves of Toona sinensis (A. Juss.) Roem. was separated into four subfractions of different polarities based on bioassay-guided method. The antioxidative activities and the related responsible compounds in T. sinensis were studied by evaluating reducing power, ferric reducing ability of plasma (FRAP) value and 1,1-diphenyl-2-picrylhydrazyl (DPPH)radical scavenging capacity of four subfractions with different polarities. The results showed that reducing power, FRAP value and DPPH radical scavenging capacity of ethyl acetate subfraction at a concentration of 50 mg/L had the strongest antioxidant activities of 467.53mg/g (ascorbic acid) Vc equivalents, 10578(μmol/L)/g FeSO47 H2O equivalents and92.84%, respectively. All these antioxidant values of ethyl acetate subfraction were much higher than BHT at the same concentration. The correlation study showed that the powerful antioxidative activity of T. sinensis was mainly resulted from high contents of flavonoids. The antioxidants mainly consist of four [JP2]flavonoid glucosides. These results laid a solid foundation for further researching and developing T. sinensis .

Key words: antioxidation, Toona sinensis (A. Juss.) Roem., bioassay-guided method, evaluation

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3