欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2010, Vol. 30 ›› Issue (3): 78-82.

• 研究报告 • 上一篇    下一篇

均匀设计法优化桦木醇超声波辅助提取工艺

丁为民1,2, 周丹3, 于涛1, 黄靖姝1, 阎秀峰1, 王洋1   

  1. 1. 东北林业大学 生命科学学院, 林木遗传育种与生物技术教育部重点实验室, 黑龙江 哈尔滨 150040;2. 哈尔滨理工大学 化学与环境工程学院, 黑龙江 哈尔滨 150080;3. 黑龙江省森林植物园, 黑龙江 哈尔滨 150040
  • 收稿日期:2009-09-22 修回日期:1900-01-01 出版日期:2010-06-30 发布日期:2010-06-30
  • 通讯作者: 王 洋(1971- ),女,教授,博士生导师,主要研究方向为植物次生代谢、植物生理生态学;E-mail:ywang1971@hotmail.com。

Optimization of Ultrasound-assisted Extraction of Betulin Using Uniform Design

DING Wei-min1,2, ZHOU Dan3, YU Tao1, HUANG Jing-shu1, YAN Xiu-feng1, WANG Yang1   

  1. 1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, College of Life Sciences, Northeast Forestry University, Harbin 150040, China;2. School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China;3. Heilongjiang Forest Botanical Garden, Harbin 150040, China
  • Received:2009-09-22 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30

PDF (PC)

696

摘要： 以95%(体积分数,下同)的乙醇为溶剂,利用均匀设计法优化了超声波辅助提取白桦树皮中桦木醇的工艺,考察了各因素对桦木醇提取率的影响,并以高效液相色谱法测定了萃取物中桦木醇的含量。结果表明,在超声波功率400W、提取时间70min、提取温度70℃、液固比40:1(mL:g)的条件下,超声波辅助提取桦木醇提取率可达到83.43%±1.89%,纯度71.20%±0.74%。与传统回流提取法相比,超声波辅助提取法提取时间短、提取率高,具有一定的应用前景。用5%的碳酸钠水溶液对原料进行预处理,可减少酸性杂质对桦木醇提取的干扰。

关键词: 桦木醇, 白桦树皮, 均匀设计, 超声波辅助提取, 高效液相色谱

Abstract: White birch (Betula platyphylla Suk.) bark, one of the important biomaterial resources, is the remains after cutting and processing of the tree. Ultrasound-assisted extraction of betulin from white birch bark using uniform design was optimized. The effects of ultrasound power, extraction temperature, extraction time and liquid-solid ratio on betulin yield was discussed, and betulin content was determined by HPLC. Betulin yield could reach up to 83.43 %±1.89% with the purity of 71.20%±0.74% under the conditions of ultrasound power 400W, extraction time 70min, extraction temperature 70℃, and liquid-solid ratio 40:1(mL:g). Comparing with the traditional reflux extraction, shorter extraction time and better yield were achieved by ultrasound-assisted extraction. Through pre-treatment with 5% sodium carbonate aqueous solution the interference of acidic impurities on the extraction of betulin could be reduced.

Key words: betulin, white birch bark, uniform design, ultrasound-assisted extraction, HPLC

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3