利用基因组DNA诱变技术选育高抑制物耐受性工业酵母菌

doi:10.3969/j.issn.0253-2417.2015.06.018

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (6): 108-112.doi: 10.3969/j.issn.0253-2417.2015.06.018

利用基因组DNA诱变技术选育高抑制物耐受性工业酵母菌

陈亮¹, 徐龙龙¹, 闫德冉², 杜凤光², 惠丰立¹

1. 南阳师范学院生命科学与技术学院, 河南南阳 473061;
2. 河南天冠企业集团有限公司车用生物燃料技术国家重点实验室, 河南南阳 473000

收稿日期:2014-09-23 出版日期:2015-12-25 发布日期:2015-12-28
通讯作者: 惠丰立,教授,硕士生导师,研究领域为酵母菌资源及菌种选育;E-mail:huifl@126.com E-mail:huifl@126.com
作者简介:陈亮(1988—),男,河南周口人,硕士生,主要从事工业酵母菌种选育工作
基金资助:
国家自然科学基金资助项目(31370073);车用生物燃料技术国家重点实验室开放课题(2013021)

Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis

CHEN Liang¹, XU Long-long¹, YAN De-ran², DU Feng-guang², HUI Feng-li¹

1. College of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China;
2. State Key Laboratory of Motor Bio-fuel technology, Henan Tianguan Group Co.Ltd, Nanyang 47300, China

Received:2014-09-23 Online:2015-12-25 Published:2015-12-28

摘要/Abstract

摘要： 通过化学诱变和基于基因组DNA 诱变的遗传重组技术,对乙醇工业酵母菌(Saccharomyces cerevisiae)的抑制物耐受性进行改造,获得了重组酿酒酵母HN-1-24,其抑制物耐受性能和发酵性能均得到了提高。重组菌株在含7 g/L 乙酸和1 g/L 糠醛的抑制物耐受性发酵培养基中,具有良好的糖转化率和乙醇产量,发酵100 g/L 葡萄糖能够产生38 g/L 乙醇。在纤维素水解液发酵培养中进行乙醇发酵,发现重组菌株的乙醇发酵效率明显快于原始菌株,发酵时间提前6 h,且发酵终点乙醇产量为37 g/L,比原始菌株HN-1提高了8.8%。

关键词: 酿酒酵母, 乙醇, 化学诱变, 遗传重组, 抑制物

Abstract: The recombinant Saccharomyces cerevisiae HN-1-24 was obtained by combination of chemical mutagenesis and genomic DNA mutagenesis-based genetic recombination methods to improve the inhibitor tolerance and fermentation performance.The recombinant strain HN-1-24 exhibited a high conversation rate of sugar to ethanol and ethanol production under a condition with 7 g/L acetic acid and 1 g/L furfural.Thus 38 g/L of ethanol could be obtained from 100 g/L of glucose.When ethanol fermentation of cellulose hydrolyzate culture was performed, the fermentation time of the recombinant strain was decreased by 6 h and the final ethanol concentration was enhanced by 8.8% as compared with the original strain.The results of this study provided a basis for the large-scale production of fuel ethanol by using lignocellulose.

Key words: Saccharomyces cerevisiae, ethanol, chemical mutagenesis, genetic recombination, inhibitor

中图分类号:

TQ35
Q813

陈亮, 徐龙龙, 闫德冉, 杜凤光, 惠丰立. 利用基因组DNA诱变技术选育高抑制物耐受性工业酵母菌[J]. 林产化学与工业, 2015, 35(6): 108-112.

CHEN Liang, XU Long-long, YAN De-ran, DU Feng-guang, HUI Feng-li. Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis[J]. Chemistry and Industry of Forest Products, 2015, 35(6): 108-112.

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利用基因组DNA诱变技术选育高抑制物耐受性工业酵母菌

Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis

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