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林产化学与工业

林产化学与工业 ›› 2013, Vol. 33 ›› Issue (6): 69-75.doi: 10.3969/j.issn.0253-2417.2013.06.013

• 1研究报告 • 上一篇    下一篇

水洗脱毒酸性蒸汽爆破玉米秸秆制备乙醇

储秋露, 杨德良, 黄阳, 余世袁, 勇强   

  1. 南京林业大学 化学工程学院, 江苏 南京 210037
  • 收稿日期:2012-12-04 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
  • 通讯作者: 勇强，教授，博士生导师，主要从事生物质资源生物降解与转化的研究；E-mail：swhx@njfu.com.cn。

Ethanol Production from Detoxified Acid Steam Exploded Corn Stover by Washing

CHU Qiu-lu, YANG De-liang, HUANG Yang, YU Shi-yuan, YONG Qiang   

  1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2012-12-04 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

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摘要： 研究了水洗对酸性蒸汽爆破玉米秸秆抑制物脱除、酶水解性能和水解液发酵性能的影响。结果表明，固液比为1：7.5（g：mL，下同）的水洗能脱除 50% 以上的碳水化合物降解产物和 70% 以上的木质素降解产物，明显提高水解糖液发酵性能和降低发酵所需的酵母用量。蒸汽爆破预处理物料经固液比为1：7.5的水洗，酶解、固液分离、蒸发浓缩后经酿酒酵母在用量为OD600 3.84下发酵48 h，170.69 g/L葡萄糖被转化为 80.67 g/L 乙醇，乙醇产率为 173 g/kg（以绝干预处理物料计，下同）。在固液比为1：7.5的水洗后的带渣发酵中，106.64 g/L 葡萄糖被转化为 54.5 g/L 乙醇，其乙醇产率为 162.17 g/kg，其酵母用量为1.0×107cfu/mL（以酶解渣计）。

关键词: 水洗, 酸性蒸汽爆破, 玉米秸秆, 乙醇

Abstract: The effects of water-washing on inhibitors removal, enzymatic hydrolysis and fermentation of acid steam-exploded corn stover were investigated. The results revealed that the washing process (solid-to-liquid ratio of 1:7.5, room temperature) removed more than 50% of the carbohydrate degradation products and more than 70% of lignin degradation products. The washing process obviously enhanced the fermentability of the concentrated enzymatic hydrolysate and decreased yeast inoculum in fermentation. The concentrated hydrolysate containing 170.69 g/L glucose, which was hydrolyzed, solid-liquid separation and concentrated from washing pretreated corn stover at solid-to-liquid ratio of 1:7.5(g:mL), was fermented by Saccharomyces cerevisiae with a low initial yeast inoculation of OD600 3.84 for 48 h. 80.67 g/L ethanol and 93.66% of ethanol yield were obtained, respectively. The corresponding ethanol production was 173.00 g based on 1 000 g dry pretreated corn stover. The whole enzymatic hydrolysate of the washed substrate at a substrate loading of 350 g/L with a yeast inoculum of 1.0×107 cfu/mL hydrolysate was fermented for 48 h. 54.50 g/L ethanol was formed from 106.64 g/L glucose, and the corresponding ethanol production was 162.17 g based on 1 000 g pretreated dry corn stover.

Key words: washing, acid steam explosion, corn stover, ethanol

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