天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性

doi:10.3969/j.issn.0253-2417.2022.01.014

林产化学与工业 ›› 2022, Vol. 42 ›› Issue (1): 101-109.doi: 10.3969/j.issn.0253-2417.2022.01.014

天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性

郁峰¹^,²(), 王志宏¹, 张光耀¹^,², 杜志云², 彭密军¹^,*()

1. 广东省科学院测试分析研究所(中国广州分析测试中心) 广东省化学危害应急检测技术重点实验室, 广东广州 510070
2. 广东工业大学生物医药学院, 广东广州 510070

收稿日期:2020-12-02 出版日期:2022-02-28 发布日期:2022-03-11
通讯作者: 彭密军 E-mail:fengyufred@163.com;pengmj163@163.com
作者简介:彭密军, 教授, 硕士生导师, 研究领域为天然产物开发与利用; E-mail: pengmj163@163.com
郁峰(1996-), 男, 四川达州人, 硕士生, 主要从事天然产物开发与利用的研究; E-mail: fengyufred@163.com
基金资助:
广东省科学院发展专项资金项目(2019GDASYL-0502003);国家自然科学基金资助项目(31660181)

Extraction of Chlorogenic Acid From Eucommia ulmoides Leaves by Natural Deep Eutectic Solvent and Its Antioxidant Activity

Feng YU¹^,²(), Zhihong WANG¹, Guangyao ZHANG¹^,², Zhiyun DU², Mijun PENG¹^,*()

1. Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Institute of Analysis, Guangdong Academy of Sciences(China National Analytical Center, Guangzhou), Guangdong Academy of Sciences, Guangzhou 510070, China
2. School of Biomedicine and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510070, China

Received:2020-12-02 Online:2022-02-28 Published:2022-03-11
Contact: Mijun PENG E-mail:fengyufred@163.com;pengmj163@163.com

摘要/Abstract

摘要：

以杜仲叶为原料，采用超声波辅助天然低共熔溶剂(NADES)提取杜仲叶绿原酸并考察其抗氧化活性。首先筛选出较优的天然低共熔溶剂体系，然后在单因素试验的基础上通过响应面优化提取工艺，并考察杜仲叶绿原酸的抗氧化活性。研究结果表明：较优的溶剂体系由甜菜碱、L-乳酸与水组成，各组分的物质的量比为1∶1∶4，低共熔溶剂中水用量为5 mol，杜仲叶(0.5 g)的绿原酸提取最佳工艺为液料比为30∶1(mL∶g)，提取温度40℃，提取时间42 min，超声波功率340 W。在此条件下绿原酸得率为31.46 mg/g，相比提取溶剂为水或者60%乙醇，分别提高了18.23%(26.61 mg/g)和14.82%(27.40 mg/g)。杜仲叶绿原酸NADES提取物体外抗氧化研究表明：提取物对DPPH·、ABTS·和·OH均有较强的清除能力，其半数抑制质量浓度(IC₅₀)分别为75.16、19.98和314.20 mg/L，对ABTS·和·OH的清除能力强于水提物和醇提物但略低于Vc。

关键词: 杜仲叶, 绿原酸, 天然低共熔溶剂, 响应面, 抗氧化

Abstract:

Chlorogenic acid was extracted from Eucommia ulmoides leaves by natural deep eutectic solvent (NADES) with ultrasonic assistance.Its antioxidant activity was investigated.Firstly, the better natural deep eutectic solvent system was screened, and then based on the single factor test, the extraction process was optimized through the response surface and the antioxidant activity of chlorogenic acid from E.ulmoides leaves was investigated.The results showed that the better solvent system was composed of betaine, L-lactic acid and water, and the ratio of each component was 1:1:4, and water consumption in eutectic solvent was 5 mol.The optimum conditions for E.ulmoides chlorogenic acid extract were liquid-to-material ratio of 30:1(mL: g), extraction temperature 40℃, extraction time 42 min, ultrasonic power 340 W.Under these conditions, the yield of chlorogenic acid was 31.46 mg/g, which increased by 18.23% and 14.82% respectively compared with the extraction solvent of water (26.61 mg/g) or 60% ethanol (27.40 mg/g).The antioxidant activity of chlorogenic acid extract from E.ulmoides leaves was studied in vitro.The results showed that the NADES extract had strong scavenging ability to DPPH·, ABTS·, and·OH, with IC₅₀ of 75.16, 19.98 and 314.20 mg/L, respectively.Its antioxidant capacity was stronger than those of water extract and alcohol extract, but slightly lower than that of Vc.

Key words: Eucommia ulmoides leaves, chlorogenic acid, natural deep eutectic solvents, response surface, antioxidant

中图分类号:

TQ35

郁峰, 王志宏, 张光耀, 杜志云, 彭密军. 天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性[J]. 林产化学与工业, 2022, 42(1): 101-109.

Feng YU, Zhihong WANG, Guangyao ZHANG, Zhiyun DU, Mijun PENG. Extraction of Chlorogenic Acid From Eucommia ulmoides Leaves by Natural Deep Eutectic Solvent and Its Antioxidant Activity[J]. Chemistry and Industry of Forest Products, 2022, 42(1): 101-109.

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参考文献 15

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甜菜碱用量/mol betaine amount	L-乳酸用量/mol L-lactic amount	水用量/mol water amount	绿原酸得率/(mg·g^-1) chlorogenic acid yield
2	1	4	21.46
2	2	4	24.48
1	1	4	31.27
1	2	4	30.94
1	3	4	30.07

方差来源 source	平方和 SS	自由度 df	均方 MS	F值 F value	P值 P value	显著性 significance
模型model	27.47	9	3.05	35.42	< 0.0001	**
X₁	2.69	1	2.69	31.22	0.0008	**
X₂	0.987	1	0.987	11.45	0.0117	*
X₃	17.43	1	17.43	202.27	<0.0001	*
X₁X₂	0.731	1	0.731	8.48	0.0226	*
X₁X₃	0.087	1	0.087	1.01	0.3485
X₂X₃	0.81	1	0.81	9.4	0.0182	*
X₁²	0.4839	1	0.4839	5.61	0.0497	*
X₂²	0.629	1	0.629	7.3	0.0306	*
X₃²	3.23	1	3.23	37.53	0.0005	**
残差residual	0.6034	7	0.0862
失拟项lack of fit	0.2401	3	0.08	0.8811	0.5223
纯误差pure error	0.3633	4	0.0908
总和cor total	28.08	16

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天然低共熔溶剂提取杜仲叶绿原酸及其抗氧化活性

Extraction of Chlorogenic Acid From Eucommia ulmoides Leaves by Natural Deep Eutectic Solvent and Its Antioxidant Activity

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序号 run	X₁ 提取时间/min extraction time	X₂ 超声波功率/W ultrasonic power	X₃ NADES中水用量/mol water amount in NADES	Y 绿原酸得率/(mg·g^-1) chlorogenic acid yield
1	10	300	4	28.23
2	30	500	2	28.26
3	30	500	6	30.51
4	30	400	4	30.78
5	10	500	4	30.07
6	10	400	2	27.10
7	50	300	4	30.20
8	30	300	6	30.40
9	30	400	4	30.58
10	30	400	4	30.10
11	10	400	6	30.15
12	50	400	6	31.05
13	30	400	4	30.14
14	30	300	2	26.94
15	30	400	4	30.59
16	50	400	2	28.59
17	50	500	4	30.34