欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2002, Vol. 22 ›› Issue (3): 54-58.

• 研究报告 • 上一篇    下一篇

芽孢杆菌产纤维素酶的研究

沈雪亮, 夏黎明   

  1. 浙江大学, 生物工程与化学工程系, 浙江, 杭州, 310027
  • 收稿日期:2001-09-10 修回日期:1900-01-01 出版日期:2002-09-30 发布日期:2002-09-30

STUDIES ON CELLULASE PRODUCTION BY BACILLUS SP.ZU-04

SHEN Xue-liang, XIA Li-ming   

  1. Department of Bioengineering and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2001-09-10 Revised:1900-01-01 Online:2002-09-30 Published:2002-09-30

PDF (PC)

776

摘要： 对芽孢杆菌(Bacillus sp.ZU-04)产纤维素酶的工艺参数进行了优化,研究结果表明:木糖渣和豆饼粉分别是该菌合成纤维素酶的适宜碳源和氮源,NaCl和KH2PO4对纤维素酶的合成具有重要作用,其适宜质量用量分别为0.5%～1.0%及0.1%,麸皮的添加可明显提高发酵液中的酶活力;3L发酵罐中的适宜发酵条件为:搅拌速度300r/min,通气量0.3L/(L·min),培养温度37℃,中性和碱性纤维素酶活力分别达到了257.6和125.6U/mL,显示了良好的工业应用前景。

关键词: 纤维素酶, 细菌发酵, 木糖渣

Abstract: Cellulase production was studied using a bacterial strain(Bacillus sp.ZU 04)screened at our laboratory.Cellulase activity could be detected from pH value 6.5 to 10.5.Corn corb residue from xylose industry was the suitable carbon source,and bean cake was the suitable nitrogen source for the strain.NaCl and KH2PO4 were important to cellulase production,and their optimal mass parts were 0.5%-1.0% and 0.1%,respectively.Cellulase activity could be improved by addition of wheat bran.Optimal culture conditions in a 3 L fermenter were: stirring speed 300 r/min,aeration 0.3 L/(L·min),and culture temperature 37℃.Neutral and alkali cellulase activities detected from the fermentation broth were 257.6 U/mL and 125.6 U/mL,respectively.The enzyme had a good application potential.

Key words: cellulase, bacterial fermentation, corn corb residue

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3