Abstract: The residue of enzymatic hydrolysis was used as carbon source to obtain poor cellulase(0.106 IU/mL)xylanases(154.67 IU/mL)by Trichoderma reesei Rut C-30,and ratio of xylanase to cellulase activity was 1 459.Compared to coarse xylan,using the residue of enzymatic hydrolysis as carbon source,the obtained xylanase activity could be increased by 67%,while cellulase activity was not increased.When using the xylanases to hydrolyze coarse xylan and the residue of enzymatic hydrolysis at 50℃,hydrolysis efficiency was highest at pH 5.0.The hydrolysis product was mainly xylose,as shown by HPLC analysis.The main component of the xylanases was exo-β-xylosidase.

Key words: Trichoderma reesei, residue of enzymatic hydrolysis, xylanases, enzymatic hydrolysis