牛尾独活叶抗红色毛癣菌活性成分研究

doi:10.3969/j.issn.0253-2417.2023.03.016

摘要/Abstract

摘要：

为了解牛尾独活叶的抗红色毛癣菌活性，以抑菌圈大小为考察指标，对比了牛尾独活不同部位、其它独活品种和常见的抗红色毛癣菌植物之间的差异，通过单因素试验和响应面法优化牛尾独活叶提取工艺，并对牛尾独活叶提取液进行溶剂萃取和化学成分预试分析。研究结果表明：伞形科不同属植物的抗红色毛癣菌活性具有差异性，牛尾独活具有较好的抑菌活性，其叶、种子、茎和根的抑菌活性差异不大。牛尾独活叶最佳抗红色毛癣菌活性的提取工艺为：乙醇体积分数25%、液料比15∶1(mL∶g)、超声波功率220 W、提取温度80 ℃、提取时间30 min，在此工艺条件下，牛尾独活叶提取液对红色毛癣菌的抑菌圈为(22±0.2)mm；牛尾独活叶提取液中抑菌成分均能较好地溶解在石油醚、乙酸乙酯和正丁醇中，其中，石油醚萃取液抑菌效果最好；化学成分预试显示牛尾独活叶具有抗红色毛癣菌的活性成分可能是多酚类和黄酮类化合物。

关键词: 牛尾独活叶, 皮肤癣病, 红色毛癣菌, 提取, 化学成分预试

Abstract:

In order to understand the anti-Trichophyton rubrum activity of the leaves of Heracleum vicinum, the difference of anti-T. rubrum activity between different parts of H. vicinum, other varieties of H. vicinum and some plants with anti-T. rubrum activity were studied using the size of inhibition zone as the index. The extraction process of H. vicinum leaves was optimized by single factor test and response surface method, and the solvent extraction and chemical composition pre-test analysis of H. vicinum leaves extract were carried out. The results showed that the anti-T. rubrum activities of different genera of Umbelliferae were different. H. vicinum had good antibacterial activity, and the antibacterial activities of leaves, seeds and roots were not significantly different. The optimal extraction conditions for the anti-T. rubrum activity of the leaves of H. vicinum was as follows: ethanol volume fraction of 25%, liquid-solid ratio of 15∶1(mL∶g), ultrasonic power of 220 W, extraction temperature of 80 ℃, and extraction time of 30 min. Under these process conditions, the inhibition zone of the extract of H. vicinum leaves against T. rubrum was (22±0.2) mm. The antibacterial components in the extract of H. vicinum leaves could be well dissolved in petroleum ether, ethyl acetate and n-butanol, where the petroleum ether extract had the best antibacterial effect. The preliminary test of chemical components showed that the active components of the leaves of H. vicinum against T. rubrum might be phenolic and flavonoid active components.

Key words: Heracleum vicinum de Boiss.leaves, dermatophytosis, Trichophyton rubrum, extraction, pre-testing of chemical composition

中图分类号:

TQ35
R756.3

吴海顺, 刘谋焱, 于华忠, 陈汇新. 牛尾独活叶抗红色毛癣菌活性成分研究[J]. 林产化学与工业, 2023, 43(3): 130-136.

Haishun WU, Mouyan LIU, Huazhong YU, Huixin CHEN. Anti-Trichophyton rubrum Active Ingredients in Leaves of Heracleum vicinum Boiss.[J]. Chemistry and Industry of Forest Products, 2023, 43(3): 130-136.

图/表 6

图1

表1

表2

图2

表3

表4

参考文献 19

1	周璐丽, 曾建国. 独活化学成分及药理活性研究进展[J]. 中国现代中药, 2019, 21 (12): 1739- 1748.
	ZHOU L L , ZENG J G . Research advances on chemical constituents and pharmacological effects of Angelica pubescens[J]. Chinese Modern Medicine, 2019, 21 (12): 1739- 1748.
2	USJAK L, NIKETIC M, DROBAC M, et al. Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe[J/OL]. Plant Systematics and Evolution, 2020, 306(1): 4[2022-03-20]. https://doi.org/10.1007/s00606-020-01645-z.
3	HASHEMINYA S M, DEHGHANNYA J. Chemical composition, antioxidant, antibacterial, and antifungal properties of essential oil from wild Heracleum rawianum[J/OL]. Biocatalysis and Agricultural Biotechnology, 2021, 31: 101913[2022-03-20]. https://doi.org/10.1016/j.bcab.2021.101913.
4	ERGENE A, TAN S, MIRICI S, et al. Antimicrobial activity of heracleum antasiaticum[C]. Antalya: 1st International Medicinal and Aromatic Plants Conference on Culinary Herbs, 2009: 267-269.
5	YU Y , DOWNIE S R , HE X , et al. Phylogeny and biogeography of Chinese Heracleum(Apiaceae tribe Tordylieae) with comments on their fruit morphology[J]. Plant Systematics and Evolution, 2011, 296 (3/4): 179- 203.
6	郑文爱, 乔凤, 唐小正. 海南地区红色毛癣菌对5种抗真菌药敏感性检测[J]. 临床皮肤科杂志, 2021, 50 (5): 270- 273.
	ZHENG W A , QIAO F , TANG X Z . Susceptibility testing of Trichophyton rubrum to 5 antifungal agents in Hainan[J]. Clinical Dermatology, 2021, 50 (5): 270- 273.
7	EICHHORN K, UHRLASS S, NENOFF P.Chronically recurrent tinea corporis due to aSuccessful terbinafine-resistant isolate from Trichophyton rubrum-therapy with itraconazole editors[J/OL]. Journal Der Deutschen Dermatologischen Gesellschaft, 2021, 19: 14455[2022-03-20]. https://doi.org/10.1111/ddg.14455.
8	余凡, 朱晓芳. 皮肤癣菌感染的治疗进展[J]. 皮肤性病诊疗学杂志, 2020, 27 (1): 57- 60.
	YU F , ZHU X F . Advances in the treatment of dermatophyte infections[J]. Journal of Dermatology and Venereology, 2020, 27 (1): 57- 60.
9	李斌, 郭鑫, 裴贵珍. 黄连萃取物体外抗红色毛癣菌活性初步研究[J]. 新疆中医药, 2019, 37 (2): 39- 41.
	LI B , GUO X , PEI G Z . Preliminary study on in vitro anti-Trichophyton rubrum activity of Coptis chinensis extract[J]. Xinjiang Traditional Chinese Medicine, 2019, 37 (2): 39- 41.
10	朱红梅, 温海, 李斌. 外用抗真菌中草药的研究进展[J]. 中国真菌学杂志, 2019, 14 (5): 316- 320.
	ZHU H M , WEN H , LI B . Research progress of topical antifungal Chinese herbal medicine[J]. Chinese Journal of Mycology, 2019, 14 (5): 316- 320.
11	刘艳霞, 杜宝中. 中草药抗菌活性研究进展[J]. 中国医药, 2021, (4): 622- 625.
	LIU Y X , DU B Z . Research progress on antibacterial activity of Chinese herbal medicine[J]. Chinese Medicine, 2021, (4): 622- 625.
12	杨晓伟, 于欢, 古少鹏, 等. 5种中药对常见皮肤癣菌抑菌效果评价[J]. 中国兽医杂志, 2016, 52 (5): 69- 71.
	YANG X W , YU H , GU S P , et al. Evaluation of bacteriostatic effect of 5 kinds of traditional Chinese medicine on common dermatophytes[J]. Chinese Journal of Veterinary Medicine, 2016, 52 (5): 69- 71.
13	廉晓芯, 朱若楠, 吴洪钦, 等. 沙果多酚的提取及组成分析[J]. 生物质化学工程, 2022, 56 (3): 29- 34.
	LIAN X X , ZHU R N , WU H Q , et al. Extraction and composition analysis of polyphenols from Malus asiatica Nakai[J]. Biochemical Engineering, 2022, 56 (3): 29- 34.
14	徐曼, 汪咏梅, 张亮亮, 等. 化香果提取物体外抑菌活性研究[J]. 生物质化学工程, 2020, 54 (2): 15- 20.
	XU M , WANG Y M , ZHANG L L , et al. In vitro antibacterial activity of extract from Platycarya strobilacea Sieb.et Zucc.[J]. Biomass Chemical Engineering, 2020, 54 (2): 15- 20.
15	贺富胤, 李凤华, 石浩, 等. 中药复配物对猕猴桃溃疡病菌抑菌机理研究[J]. 四川农业大学学报, 2022, 40 (6): 872- 877.
	HE F Y , SHI H , LI F H , et al. Study on the antibacterial mechanism of Chinese medicine compound on kiwifruit ulcer pathogen[J]. Journal of Sichuan Agricultural University, 2022, 40 (6): 872- 877.
16	张正海, 姬妍茹, 杨庆丽, 等. 响应面法优化工业大麻叶抑制金黄色葡萄球菌物质超声提取工艺[J]. 中国麻业科学, 2019, 41 (2): 71- 78.
	ZHANG Z H , JI Y R , YANG Q L , et al. Optimization of ultrasonic extraction process of industrial hemp leaves inhibiting Staphylococcus aureus by response surface method[J]. China Jute Science, 2019, 41 (2): 71- 78.
17	谢华松, 罗宝平, 黄亚燕, 等. 钝药野木瓜化学成分预试研究[J]. 山东化工, 2022, 51 (9): 119- 121.
	XIE H S , LUO B P , HUANG Y Y , et al. Preliminary study on the chemical constituents of chaenomeles speciosa[J]. Shandong Chemical Industry, 2022, 51 (9): 119- 121.
18	林大都, 李俊芳, 翟明, 等. 禺毛茛化学成分预试研究[J]. 广东化工, 2021, 48 (11): 17- 18.
	LIN D D , LI J F , ZHAI M , et al. Preliminary study on the chemical constituents of ranunculus yuyuensis[J]. Guangdong Chemical Industry, 2021, 48 (11): 17- 18.
19	李伟业, 吴海顺, 于华忠. 七种独活、当归药材中蛇床子素及总香豆素含量比较[J]. 亚太传统医药, 2021, 17 (4): 36- 40.
	LI W Y , WU H S , YU H Z . Comparison of osthole and total coumarin content in seven kinds of radix angelicae pubescentis and radix angelicae sinensis[J]. Asia-Pacific traditional medicine, 2021, 17 (4): 36- 40.

方差来源variance	平方和sum of squares	自由度freedom	均方mean square	F值F value	P值P value	显著性significant
模型model	114.31	9	12.70	43.79	<0.000 1	**
X₁	2.23	1	2.23	9.64	0.027 7	*
X₂	1.97	1	1.97	8.53	0.035 1	*
X₃	3.47	1	3.47	15.03	0.010 6	*
X₁X₂	3.50	1	3.50	15.14	0.010 4	*
X₁X₃	0.062 5	1	0.062 5	0.270 6	0.656 6
X₂X₃	3.55	1	3.55	15.38	0.010 0	*
X₁²	15.59	1	15.59	60.80	0.000 2	**
X₂²	7.41	1	7.41	27.52	0.001 5	**
X₃²	68.61	1	68.61	282.80	<0.000 1	**
残差residual	2.03	7	0.290 1
失拟项lack of fit	1.62	3	0.539 0	5.21	0.072 3
纯误差pure error	0.413 5	4	0.103 4
总误差cor total	116.34	16

萃取物extract	分级萃取/mm fractional diffusion extraction	逐级萃取/mm polarized extraction
石油醚PE	26.91	26.91
乙酸乙酯EA	32.52	25.64
正丁醇n-butanol	35.35	22.59
水H₂O	26.84	28.52

样品specimen	生物碱alkaloid	多酚类polyphenols	甾体皂苷steroid saponins	萜类terpenoids	黄酮及其苷类flavonoids	有机酸organic acids	蒽醌及其苷类anthraquinone	香豆素内酯及其苷类coumarin lactone
醇提液alcohol	+	+	+	+	+	-	+	+
石油醚萃取液petroleum	-	+	+	+	+	-	-	-

[1]	姜萍, 曲肼靓. 东北红豆杉枝叶中紫杉宁提取分离及其抗肿瘤活性[J]. 林产化学与工业, 2023, 43(3): 137-144.
[2]	蒋剑春. 林源饲料和添加剂技术现状及展望[J]. 林产化学与工业, 2023, 43(1): 1-14.
[3]	沈红, 张昌伟, 陈虹霞, 陶冉, 蒋建新, 王成章. 银杏叶色素类化合物的提取分离及结构鉴定[J]. 林产化学与工业, 2023, 43(1): 79-88.
[4]	安容苗, 袁婷, 郭雪峰. 簕竹属竹叶中黄酮类成分分析及其抗氧化活性[J]. 林产化学与工业, 2023, 43(1): 97-103.
[5]	姜萍, 曲肼靓, 贾雨欣, 孙智超, 刘思盈, 王飞. 东北红豆杉枝叶中原花色素的提取分离及其糖尿病抑制活性[J]. 林产化学与工业, 2022, 42(6): 108-116.
[6]	何旭华, 石志娇, 彭小伟, 阚欢, 赵平, 刘云. 云南金花茶叶不同极性提取物的抗氧化活性分析[J]. 林产化学与工业, 2022, 42(5): 61-68.
[7]	黄维, 郭梦云, 王玉启, 林金垒, 张宇翔, 邹双全. 圆齿野鸦椿果皮多糖的提取及其抗炎活性研究[J]. 林产化学与工业, 2021, 41(3): 77-84.
[8]	陈淑琴, 叶燕燕, 全颖萱, 李林海, 倪林, 邹双全. 圆齿野鸦椿籽油提取工艺及脂肪酸组分分析[J]. 林产化学与工业, 2021, 41(1): 53-59.
[9]	蒋一苇, 郭娜, 刘志国, 赵春建, 付玉杰, 王希清. 蓝靛果籽油提取工艺优化及其脂肪酸组成分析[J]. 林产化学与工业, 2020, 40(6): 30-36.
[10]	房仙颖, 谢莹莹, 章祎唯, 曹福亮, 赵林果. 银杏酸毒副作用及朴树提取物对其减毒效果研究[J]. 林产化学与工业, 2020, 40(6): 99-106.
[11]	童天娇,刘军海,刘艳,曹瑞,魏玲玲. 杨梅叶中杨梅素的提取及抗氧化性研究[J]. 林产化学与工业, 2020, 40(5): 114-120.
[12]	王豪缘,钞运竹,李冬梅,胡滨. 微波和超声波辅助提取桃仁油工艺的比较研究[J]. 林产化学与工业, 2019, 39(6): 44-52.
[13]	陈虹霞,王成章,叶建中,周昊,陶冉,李文君. 漆树提取物的热稳定性及分解动力学研究[J]. 林产化学与工业, 2019, 39(6): 81-87.
[14]	姜萍,赵雅洁,包信业,王飞,段占泉,王茂霖. 红豆杉枝叶中总生物碱的提取及其抗肿瘤活性研究[J]. 林产化学与工业, 2019, 39(6): 115-122.
[15]	王晓杰,黄立新,张彩虹,谢普军,邓叶俊,刘璐婕. 油橄榄叶提取物的制备及理化性质研究[J]. 林产化学与工业, 2019, 39(4): 83-90.