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林产化学与工业 ›› 2011, Vol. 31 ›› Issue (1): 81-85.

• 研究报告 • 上一篇    下一篇

利用汽爆玉米芯酶解液发酵生产类胡萝卜素的研究

唐棠, 杨洲平, 黄乾明   

  1. 四川农业大学 生命科学与理学院, 四川 雅安 625014
  • 收稿日期:2010-09-02 修回日期:1900-01-01 出版日期:2011-02-28 发布日期:2011-02-28
  • 通讯作者: 黄乾明,教授,研究领域为微生物生物化学;E-mail:hqming@sicau.edu.cn。

Study on Fermentation of Hydrolysate from Steam-exploded Corn Corbs to Produce Carotenoid

TANG Tang, YANG Zhou-ping, HUANG Qian-ming   

  1. College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China
  • Received:2010-09-02 Revised:1900-01-01 Online:2011-02-28 Published:2011-02-28

摘要: 研究了汽爆玉米芯酶水解及酶解液发酵生产类胡萝卜素。对影响酶解各因素考察表明:在最适酶解条件下,即温度50℃、pH值4.8、底物质量浓度50g/L、加酶量48FPU/g、酶解时间60h,还原糖质量浓度达34.85g/L。利用均匀设计对发酵条件进行了优化,最优发酵条件为:装液量23.91mL,pH值4.85,接种量9.46%(体积分数),摇床转速190r/min,核黄素2mg/L,在此条件下类胡萝卜素产量可达11.24mg/L。

关键词: 玉米芯, 酶解, 红酵母, 类胡萝卜素

Abstract: Fermentation of hydrolysate from steam-exploded corn corbs to produce carotenoid was investigated. Factors affecting enzymatic hydrolysis were studied. The optimum conditions were as follows:temperature 50℃, pH value 4.8, substrate mass concentration 50g/L and enzyme loading 48 FPU/g. After 60 h hydrolysis, 34.85g/L of reducing suqars were obtained. Fermentation process for carotenoids production was optimized by a uniform design experiment. The optimum fermentation parameters were as follows: volume charge 23.91mL, initial pH value 4.85, inoculum dosage 9.46%(volume fraction), rotation speed 190r/min, lactoflavin 2mg/L. The production of carotenoid could reach 11.24mg/L.

Key words: corn cob, enzymatic hydrolysis, red yeasts Rhodotorula, carotenoid

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