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林产化学与工业

林产化学与工业 ›› 2012, Vol. 32 ›› Issue (5): 29-33.

• 1研究报告 • 上一篇    下一篇

低能离子注入法诱变选育纤维素酶产生菌的研究

张宁, 蒋剑春, 杨静, 卫民, 赵剑, 童娅娟   

  1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局 林产化学 工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏 南京 210042
  • 收稿日期:2012-04-10 修回日期:1900-01-01 出版日期:2012-10-30 发布日期:2012-10-30
  • 通讯作者: 蒋剑春,研究员,博士,博士生导师,从事林产化学加工和生物质能源开发技术研究;E-mail:bio-energy@163.com。

Mutation Breeding of Cellulase Producing Strain by Low Energy Ion Implantation

ZHANG Ning, JIANG Jian-chun, YANG Jing, WEI Min, ZHAO Jian, TONG Ya-juan   

  1. Institute of Chemical Industry of Forest Products,CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.of Forest Chemical Engineering,SFA;Key Lab.of Biomass Energy and Material,Jiangsu Province, Nanjing 210042, China
  • Received:2012-04-10 Revised:1900-01-01 Online:2012-10-30 Published:2012-10-30

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摘要： 以纤维素酶产生菌里氏木霉(Trichoderma reesei)为实验菌株,研究了低能N+注入对T.reesei存活率、菌体总抗氧化能力(T-AOC)、蛋白质含量以及产酶能力的影响。结果表明, T.reesei经N+注入后存活率曲线呈现"双马鞍型",这与离子注入典型的"马鞍型"曲线不同。菌体总抗氧化能力与注量之间的关系曲线同样在1.50×1016和2.50×1016 cm-2注量处呈现两个峰,与存活率曲线的变化趋势一致。由此推测,离子注入微生物所诱导的总抗氧化能力的强弱变化很有可能决定微生物的存活情况。实验通过诱变筛选得到3株纤维素酶高产菌株150-1, 150-2和250-6,高产菌株纤维素酶的平均活力比出发菌株提高 24.3 %,均表现出很强的发酵积累纤维素酶的能力,尤其在发酵后期更为突出。

关键词: 里氏木霉, 纤维素酶, 氮离子注入, 双马鞍型曲线

Abstract: Trichoderma reesei was used as experimental model, and the effects of low-energy N+ implantation on its survival rate, total antioxidant capability (T-AOC), protein concentration and cellulase yield were investigated. The results showed that the dose-survival rate curve took "double saddle shape" with increasing N+ implantation doses. It was different from the typical "saddle shape" curve of ion beam implantation. The curve of T-AOC and dose also has two peaks at the doses of 1.50×1016 and 2.50×1016 cm-2, which were exactly the same as that of the survival rate curve. So it was speculated that the changes of T-AOC probably decided the microbe survival situation after being implanted by low-energy N+. Three cellulase high yielding strains T.reesei 150-1、150-2 and 250-6 were obtained and the average production of cellulase was raised by 24.3% in shaking flasks. Comparing with the control strain, the mutants were potent in accumulating cellulase especially in the later stage.

Key words: Trichoderma reesei, cellulose, N+ implantation, double saddle shape curve

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