纤维素酶基因表达研究进展

doi:10.3969/j.issn.0253-2417.2014.05.028

摘要/Abstract

摘要： 获得更高生产性能的菌株是降低纤维素酶在纤维乙醇、纸浆造纸、纺织等领域使用成本的主要解决途径。总结了影响宿主菌（里氏木霉和毕赤酵母）高效表达纤维素酶基因的多种因素，如里氏木霉转录调控、启动子优化、外源酶基因与毕赤酵母表达的适配性、启动子拷贝数及酶基因拷贝数等，以期为纤维素酶高产菌株的构建提供有益的参考。

关键词: 纤维素酶, 转录调控, 基因表达, 里氏木霉, 毕赤酵母

Abstract: Acquisition of a more excellent producing strain of cellulase is the major approach to decrease the cost of application in the field of cellulosic ethanol, pulp and paper, textile and so on. In order to review some valuable information on the construction of high yielding strain of cellulase, a variety of factors, including regulation of cellulase in Trichorderma reesei, optimization of promoter, the compatibility between cellulase gene and the host, the copy number of promoter and expressed gene, were discussed. Their influence on the expression of cellulse gene in T.reesei or Pichia pastoris was summarized.

Key words: cellulase, transcriptional regulatinon, gene expression, Trichoderma reesei, Pichia pastoris

中图分类号:

TQ35

何敏超, 许敬亮, 袁振宏, 孔晓英, 张宇, 陈小燕, 梁翠谊. 纤维素酶基因表达研究进展[J]. 林产化学与工业, 2014, 34(5): 169-174.

HE Min-chao, XU Jing-liang, YUAN Zhen-hong, KONG Xiao-ying, ZHANG Yu, CHEN Xiao-yan, LIANG Cui-yi. Research Progress in the Expression of Cellulase[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 169-174.

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