Abstract: A Bacillus strain was isolated from waste pulp.The strain had high endo-β-glucanase producing ability proved by the Congo red dying method on the culture plates.Optimal growing conditions of the strain were 35～37℃,pH 9.0～10.0.Results in shaking flask culture showed that high cellulase activities(CMCase)could be detected in both neutral and alkali conditions from the fermentation broth,and the neutral and alkali cellulase activities were 69.4U/mL and 53.6U/mL,respectively.The optimal conditions for the enzymatic reaction were 50℃,and pH value neutral to alkali.Ca⁺²,Mn⁺² were the activators and Pb⁺²,Hg⁺² were the inhibitors of the enzyme.This cellulase preparation had a good application potential in the textile biofinishing and detergent industry.

Key words: neutral cellulase, alkali cellulase, bacteria