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林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 73-76.

• 研究报告 • 上一篇    下一篇

水竹叶总黄酮的提取及纯化工艺研究

陈文英, 王成章, 叶建中, 陈虹霞   

  1. 中国林业科学研究院 林产化学工业研究所;国家林业局 林产化学工程重点开放性实验室, 江苏 南京 210042
  • 收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20
  • 通讯作者: 王成章,研究员,博士,硕士生导师,从事天然产物的化学加工、保健产品及药物开发;E-mail:wangczlhs@sina.com。

Studies on Extraction and Purification of Flavonoids from Phyllostachys heteroclata Oliver

CHEN Wen-ying, WANG Cheng-zhang, YE Jian-zhong, CHEN Hong-xia   

  1. Institute of Chemical Industry of Forest Product, CAF;Key and Open Lab.on Forest Chemical Engineering, SFA, Nanjing 210042, China
  • Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

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摘要： 以水竹叶为原料,采用水浴和超声波两种提取方法,通过单因素与正交试验确定了水竹叶总黄酮提取的最佳条件,并筛选了不同树脂对总黄酮的吸附和解吸附特性.实验结果表明:水浴提取最佳条件为:温度50℃,提取时间2.0 h,乙醇体积分数60%,固液比1:20(g:mL,下同);总黄酮得率1.65%.影响得率的因素为:固液比>乙醇体积分数>提取温度>提取时间.超声波提取最佳条件为:2.0 g水竹叶,超声波作用时间30 min,乙醇体积分数60%,固液比1:15;总黄酮得率为1.27%.影响得率的因素为:超声波作用时间>乙醇体积分数>固液比.AB-8树脂对总黄酮纯化具有较好效果,体积分数为70%的乙醇是较好的解吸剂.

关键词: 水竹叶, 总黄酮, 正交试验, 提取纯化

Abstract: Flavonoids from Phyllostachys heteroclata Oliver were extracted by methods of waterbath and ultrasonic extractions.The optimum extraction conditions were obtained by single factor and orthogonal tests.Different kinds of macro-reticular resins were tested for purification.The results showed that the yield of flavonoids was 1.65% by waterbath extraction under the conditions of 60% ethanol, 50℃,1:20 solid-liquid ratio and 1.5 h.Order of the factors which affected waterbath extraction was: ratio of soild to liquid>concentration of ethanol solution>extraction temperature>extraction time. The yield of flavonoids was 1.27% by ultrasonic extraction under the conditions of 60% ethanol, 1:15 solid-liquid ratio and 30 min. Order of the factors which affected ultrasonic-assisted extraction was: extraction time>volume fraction of ethanol solution>ratio of soild to liquid.It was found that AB-8 macro-reticular resin was superior for the purification of flavonoids,and 70% ethanol was a good eluant.

Key words: Phyllostachys heteroclata Oliver leaves, flavonoids, orthogonal test, purification

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