关键词: 麦糟, 固态发酵, 纤维素酶

Abstract: Cellulase production was carried out by solid-state process with Trichoderma reesei Rut C-30 when distiller's wheat was employed.In the trays, the optimized koji temperature was as follows:before 48h koji temperature should be controlled between 27～30℃, after 48h below 35℃, and maximal FPA activity of 611U/g was achieved.When pH value increased back to 6.0～6.2, FPA activity ceased to increase or declined.Based on the investigated data pH control strategy was proposed to be carried out when pH rised back to 4.3～4.5 for the first time.Spraying of inorganic acid into substrate, such as HCl, could counteract the increase of pH during cultivation.With pH control technology, 43.7% improvement of FPA activity was estimated and the results clearly illustrated that pH control enhanced cellulase production.With koji temperature and pH control technology, FPA activity of 508U/g koji(about 2120 U/g cellulose) was obtained.The pH control technology could be carried out in large scale solid state fermentation of cellulase.

Key words: distiller′s wheat, solid-state fermentation, cellulase